摘要目的 探讨β2肾上腺素受体在婴儿血管瘤发病机制中的作用.方法 婴儿血管瘤内皮细胞分为普萘洛尔组及异丙肾上腺素组.普萘洛尔组再分为10、15、20 μg/ml组、空白组及二甲基亚砜(DMSO)组5组,异丙肾上腺素组再分为5、10、20 μg/ml组、空白组4组.血管瘤内皮细胞与这些组作用24 h、48 h,用酶联免疫吸附法分别测定每浓度细胞培养上清液中β2肾上腺素受体的表达.结果 作用24 h后,15 μg/ml、20 μg/ml组普萘洛尔与空白组和DMSO组比较,β2肾上腺素受体的表达下降,差异有统计学意义(均P＜ 0.05).48 h后,普萘洛尔组各浓度培养的细胞,β2肾上腺素受体表达较空白组下降,差异有统计学意义(均P＜ 0.05).而异丙肾上腺素组,作用24 h后,10μg/ml、20 μg/ml组的异丙肾上腺素较空白组β2肾上腺素受体的表达增高,差异有统计学意义(均P＜0.05).48 h后,20 μg/ml组异丙肾上腺素培养的细胞上清液中的β2肾上腺素受体表达较空白组高,差异有统计学意义(均P＜ 0.05).结论 普萘洛尔对血管内皮细胞表面的β2肾上腺素受体表达的影响下调,而异丙肾上腺素作用上调.

abstractsObjective To explore the role of beta-2 adrenergic receptor in the pathogenesis of infantile hemangioma.Methods In vitro cultured infantile hemangioma endothelial cells were divided into propranolol and isoproterenol groups.The propranolol groups were further classified into 5 groups to be treated with propranolol solutions at concentrations of 10,15,20 μg/ml,EGM-2 medium (blank control group 1),and EGM-2 medium containing 0.16％ DMSO (DMSO group) respectively,while the isoproterenol groups were classified into 4 groups to be treated with isoproterenol solutions at concentrations of 5,10,20 μg/ml and EGM-2 medium (blank control group 2) respectively.After 24-and 48-hour treatment,enzyme-linked immunosorbent assay (ELISA) was performed to measure the expression of beta-2 adrenergic receptor in cell culture supernatants in the above groups.Results After 24-hour treatment,15-μg/ml and 20-μg/ml propranolol groups showed significantly decreased expression of beta-2 adrenergic receptor compared with blank control group 1 and DMSO group (all P ＜ 0.05).After 48-hour treatment,all the propranolol groups showed significantly decreased expression of beta-2 adrenergic receptor compared with the blank control group 1 (all P ＜ 0.05).However,the expression of beta-2 adrenergic receptor was significantly higher in the 10-and 20-μg/ml isoproterenol groups than in the blank control group 2 after 24-hour treatment (all P ＜ 0.05),and higher in the 20-μg/ml isoproterenol group than in the blank control group 2 after 48-hour treatment (P ＜ 0.05).Conclusion Propranolol can down-regulate the expression of beta-2 adrenergic receptor on the surface of vascular endothelial cells,while isoproterenol can up-regulate its expression.