摘要目的探讨术前区域动脉灌注化学药物治疗(化疗)对结直肠癌细胞生长周期的影响。方法采用Seldinger技术，经股动脉插管将化疗药注入癌灶供血动脉。化疗结直肠癌30例，术后10d进行结直肠癌根治术。用免疫组织化学方法测定p16蛋白和Rb蛋白的表达情况，与未行化疗的对照组进行比较。结果术前区域动脉灌注化疗组p16蛋白表达标记指数（LI）为(35±19)%；对照组为(16±8)%。化疗组p16蛋白表达明显高于对照组，P＜0.01。术前区域动脉灌注化疗后癌细胞中Rb蛋白表达的LI为（48±11）%，明显高于对照组〔LI为（38±10）%〕，P＜0.01。p16蛋白与Rb蛋白表达与Dukes分期显著相关，B期表达较C、D期为高。结论术前区域动脉灌注化疗可通过上调p16与Rb蛋白表达，在G1/S限制点发挥抑制细胞周期进入S期的作用。

abstractsObjective　To observe the influence of preoperative regional chemotherpy on colon cancer cell cycle.Methods　30 colorectal cancer patients received intraarterial chemotherapy 10 days before the radical resection.The expression of p16 and Rb protein was examined by immunohistochemistry.Result was compared with the control group.Results　Labeling index (LI) of p16 protein was (35±19)% in treatment group,while in control group L1 was (16±8)%,(P＜0.01).LI of Rb protein was (48±11)% in treatment group and (38±10)% in control group (P＜0.01).The expression of p16 and Rb protein were significantly correlated with the Dukes staging,especially with stage B.Conclusion　p16 and Rb protein make a negative regulation in the P16/CDK/cyclinD/Rb/E2F pathway.Preoperative regional chemotherapy may arrest the cell cycle in the G1/S checkpoint by up-regulating the expression of P16 and Rb.