摘要目的 探讨livin基因在肝癌组织中的表达及乙型肝炎病毒X蛋白(hepatitis B virus Xprotein,HBx)对其表达的影响.方法 采用免疫组化染色法检测10例正常肝组织和30例肝癌组织中livin基因的表达.建立稳定转染HBx基因的L02(L02-X)细胞系,采用Real-time PCR和Western blot检测L02-X)细胞系转染HBx基因前后livin基因表达的变化.结果 Livin基因在肝癌组织的表达明显高于正常肝组织,且livin基因的表达与肝癌的大小、病理分级无明显相关性(t=1.688,P=0.103;γ=-0.137,P=0.471;P＞0.05),与门静脉癌栓有关(t=2.24,P=0.033;P＜0.05).转染HBx基因后livin基因mRNA表达水平上调(t=6.73,P＜0.05).结论 livin基因高表达引起的凋亡抑制可能在肝癌的发生、发展中起重要作用,HBx基因可以在转录水平上调其表达.

abstractsObjective To investigate the expression of livin in hepatocarcinoma tissues and its relationship with hepatitis B virus X protein. Methods Immunohistochemistry was used to detect the expression of livin in 30 cases of hepatic carcinoma tissues and 10 cases of normal liver tissues. Stable L02 cell hne tansfected with HBx was established. Real-time PCR and Western blot were used to observe the expression of livin gene before and after the HBx transfeetion. Results The protein expression of livin was higher in hepatocarcinoma tissues than in normal tissues, and correlated with the exist of portal vein cancer embolus (t = 2.24, P = 0.033 ; P<0.05), but it was not correlated with cancer size or classification pathologically(t = 1.688, P = 0.103 ; γ = - 0.137, P = 0.471 ; P＞0.05). Livin Mrna expression was up-regulated after the HBx gene transfection (t = 6.73, P<0.05). Conclusions Apoptosis inhibition induced by livin up-regulation may play a role in pathogenesis and development of hepatocarcinoma. HBx up-regulates livin expression at transcriptional level.