摘要目的 探索N-3,4-二甲基氧基肉桂酰-邻氨基苯甲酸(3,4-DAA)通过吲哚胺2,3双加氧酶(IDO)高表达介导小鼠皮肤移植免疫负向调节的作用及其机制.方法 建立小鼠皮肤移植模型,收集受鼠脾脏淋巴细胞及血清,检测白细胞介素2(IL-2)和γ干扰素(IFN-γ)含量;流式细胞仪测定T淋巴细胞各亚群;RT-PCR检测IDO信使RNA(mRNA)基因表达水平.结果 3,4-DAA对小鼠脾脏淋巴细胞增殖有显著抑制作用,并呈现剂量效应关系.3,4-DAA可显著抑制小鼠IL-2和IFN-γ的产生(P＜0.05).3,4-DAA处理组CD3+、CD4+、CD8+T淋巴细胞百分率呈现下降趋势,同时调节性T淋巴细胞百分率增加,但差异无统计学意义(P＞0.05).3,4-DAA处理组IDO mRNA基因表达较对照组均显著增高(P＜0.05).结论 3,4-DAA可抑制T淋巴细胞增殖,并通过上调IDO高表达下调移植物免疫应答反应,显示出免疫负向调节的效应.

abstractsObjective To investigate role of N-(30,40-dimethoxycinnamonyl) anthranilic acid (3,4-DAA) in mediating the negative immune regulation by indoleamine 2,3-dioxygenase in mice skin transplantation.Method The mice skin transplant model was established.T lymphocytes were isolated from mice splenocytes,and serum was collected.ELISA was applied to detect the IL-2 and IFN-γ concentrations in culture supernates of mice spleen lymphocytes and serum.Flow cytometry was applied to examine the phenotypes of T lymphocytes and RT-PCR to analyze the IDO mRNA transcription.Results T lymphocyte proliferation was significantly decreased by 3,4-DAA and the concentrations of IL-2 and IFN-γ were apparently decreased in 3,4-DAA group as compared with those in control group.The percentages of CD3 +,CD4 + and CD8 + T lymphocytes were lower,and the percentage of CD4 + CD25 + T lymphocytes was higher in 3,4-DAA disposal group than in control group,but there was no significant difference.IDO mRNA expression showed obvious increase in 3,4-DAA group as compared with control group.Conclusion 3,4-DAA can up-regulate the IDO expression to induce the increasing metabolism of tryptophan,subsequently inhibiting T cell proliferation and showing specific property of negative immune regulation.