摘要Single-cell transcriptome sequencing has been a rapidly developing and powerful biological tool in recent years,and it plays a vital role in describing tissue development,cell heterogeneity,stress response,etc. Cerebrovascular disease is one of the leading causes affecting human health in the world. Thus,it is import-ant to understand the characteristics of cerebrovascular structure,function,and environmental response. Notably,single-cell transcriptome sequencing provides deeper in-sights into cerebrovascular research in health and disease states. This article will briefly introduce the basic struc-ture and function of cerebrovascular endothelial cells (ECs),summarize the current research and new findings on cerebrovascular ECs at the single-cell transcriptome level,and discuss the challenges in this field.