摘要Objective:To investigate the regulato-ry role of cyclic adenosine monophosphate responsive element binding protein (CREB)/brain-derived neuro-trophic factor (BDNF) signaling pathway in acute sleep deprivation (SD)-induced anxiety-like behavior mice (SD group) to study the mechanism of anxiety-like be-havior better. Methods:The SD chamber was used to deprive the mice of sleep,and the anxiety-like behavior of the mice was verified using an open field test (OFT),elevated plus maze (EPM),forced swim test (FST),and tail suspension test (TST). Finally,proteins were detect-ed by Western blotting. Result:OFT showed that the active distance and the time of stay in the central area were significantly reduced (P<0.05). EPM showed that the time and number of open arms in the SD group were significantly lower than in the control group (P<0.05). The FST showed that the forced swimming immobility time of the SD group was significantly lower than that of the control (P<0.05). Moreover,the TST showed that the immobility time of the tail suspension experi-ment in the SD group was significantly higher than that in the control group (P<0.05). Conclusion:Acute SD can regulate anxiety-like behavior in mice through the CREB/BDNF signaling pathway.