摘要目的 探索特异性siRNA靶向干扰恶性胶质瘤U251细胞中INTα_vβ_3表达后对细胞生长的作用.方法 将INTα_vβ_3特异性siRNA经脂质体(LipofectamineTM~(2000))转染U251细胞.RT-PCR、免疫细胞化学和Western blot法检测INTα_vβ_3蛋白表达.MTT检测干扰后细胞增殖变化.结果 INTα_vβ_3特异siRNA对U251细胞增殖有明显抑制作用.siRNA组、脂质体组与对照组各时间点的结果 差异有统计学意义.免疫细胞化学对照组和脂质体组均见INTα_vβ_3呈绿色表达,siRNA组表达降低.Western blot法和RT-PCR结果 证实转染特异siRNA后的细胞在蛋白及mRNA水平均能抑制INTα_vβ_3的表达.结论 特异性siRNA可干扰INTα_vβ_3在脑胶质瘤U251细胞中的表达并抑制细胞增殖.

abstractsObjective Explore the specificity of siRNA interference targeting malignant glioma U251 cells, INTα_vβ_3 expression of cell growth after the role. Method U251 cells were transfected with INT α_vβ_3 specific siRNA by lipofectamine(LipefectamineTM~(2000)). RT-PCR, immune ceils staining and Western blot were used to determine the expression of INTα_vβ_3. Cell proliferation after the detection of changes in interference was tested by MTT. Results INTα_vβ_3 specific siRNA shown to inhibit U251 cell proliferation. There were statistical significance between siRNA group, liposome group and the control group at different time points. Green INTα_vβ_3 expression was found in control group and lipesome groups, and reduced in siRNA group. Western blotting and RT-PCR results shown that the expression of integrin could be inhibited by α_vβ_3 transfection in the protein and mRNA level Conclusions Integrin-specific siRNA INTα_vβ_3 can inhibit the U251 glioma cell proliferation.