摘要目的 观察硼中子俘获疗法对C6鼠胶质瘤细胞增殖、凋亡及迁移的影响.方法 C6细胞体外培养,加含硼培养液,医院中子照射器照射.实验分为三组:3 Gy照射组(Ⅰ组)、6 Gy照射组(Ⅱ组)、对照组(Ⅲ组).采用MTT法检测细胞增殖,绘制照射后1～7d细胞生长曲线;应用流式细胞仪检测照射72 h后细胞凋亡率;采用划痕试验观察照射72 h后细胞迁移情况.结果 中子照射后7d三组细胞的存活数分别为(7.90±0.23)×105个/孔、(5.40 ±0.35)×105个/孔和(9.42±0.23)×106个/孔,Ⅰ组和Ⅱ组分别与Ⅲ组差异具有统计学意义(P＜0.01);中子照射后72 h三组细胞凋亡率分别为(37.3±0.7)％、(84.7±2.3)％和(23.1±1.4)％,三组之间两两比较差异具有统计学意义(P＜0.01);中子照射72 h后细胞迁移能力明显降低.结论 硼中子照射可有效抑制胶质瘤细胞增殖,诱导其凋亡并降低其迁移能力.

abstractsObjective To investigate the proliferation,apoptosis and migration of boron neutron capture therapy(BNCT) for C6 rat glioma cell-line.Methods The C6 cells,cultured by 10 B-DMEM and with irradiation by in-hospital neutron irradiator (IHNI),were divided into three groups:BNCT 3 Gy (Group Ⅰ),BNCT 6 Gy (Group Ⅱ),untreated control (Group lⅢ).The MTT assay was used to indentified the cell proliferation and draw the cell growth curve at 1-7 days of post-irradiation.The apoptosis rate was detected by flow cytometer (FCM) after 72 h of irradiation.Cell scratch test was performed to investigate the cell migration at 72 h of post-irradiation.Results One-well cell number of each group accounts to (7.90 ± 0.23) × 105,(5.40 ± 0.35) × 105 and (9.42 ± 0.23) × 106.Both Group Ⅰ and Ⅱ were with significant difference from Group Ⅲ(P ＜0.01).The apoptosis rates of three groups after 72 h of irradiation were (37.3 ± 0.7) ％、(84.7 ± 2.3) ％ and (23.1 ± 1.4) ％,each group showed statistic significancant difference to the others (P ＜ 0.01).Moreover,the C6 cells showed decreased migrating ability at 72 h after irradiation.Conclusions By using boron neutron capture therapy,it can effectively inhibit glioma cells' proliferation,induce apoptosis and weaken its migrating ability.