摘要目的 探讨脑肿瘤干细胞(BTSCs)移植瘤组织中的肿瘤细胞、血管内皮细胞的起源问题,旨在阐明BTSCs在脑肿瘤组织重构中所起的作用. 方法 BTSCs球体接种到NC系裸小鼠右尾状核,在出现恶病质时处死,取移植瘤组织常规石蜡包埋、切片后进行HE染色和人类白细胞抗原(HLA)染色,在光学显微镜下观察. 结果 HE染色见肿瘤细胞广泛播散在宿主的脑组织中.HLA染色显示存在肿瘤细胞直接构成的血管. 结论 在BTSCs的裸小鼠移植瘤中.肿瘤细胞在宿主脑内广泛分布并构成了血管;BTSCs可能转分化成肿瘤血管细胞,在脑胶质瘤组织重构中起重要作用.

abstractsObjective To investigate the origin of tumor cells and vascular endothelial cells in intraeranial brain tumor stem cell (BTSC) xenografls and elucidate the role of BTSCs in brain tumor tissue remodeling. Methods BTSC spheres were injected into the right candate nucleus of nude mice, and the mice were sacrificed after the occurrence ofcachexia to obtain the tumor tissue. Routine paraffin embedding, slicing, HE staining and human leucocyte antigen (HLA) staining of the tumor tissues were performed for observation of the tumor tissues under optical microscope. Results HE staining displayed dissemination growth of the tumor cells in the host brain tissue. HLA staining revealed the presence of blood vessels constituted directly by the tumor cells. Conclusion In the intracranial xenografts of BTSCs, the tumor cells are extensively distributed and constitute the blood vessels. The BTSCs play an important role in glioma tissue remodeling by differentiating into vascular endothelial cells in the tumors.