摘要目的 观察母源性BDE-209暴露后仔鼠海马组织结构形态及海马Ca2+/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)含量的变化.方法 3月龄Wistar大鼠白妊娠0 d起按照随机数字表法分为实验组和对照组(E组),实验组母鼠在妊娠期及哺乳期给予不同剂量BDE-209(100、300、600、1200mg/kg)胃灌,分别为A组、B组、C组、D组;E组胃灌等量花生油,21 d仔鼠断乳后各组选取15只雄鼠,HE染色后光镜观察仔鼠海马组织的变化,ELISA法检测海马CaMKⅡ含量.结果 HE染色后光镜下观察发现C、D组仔鼠海马组织形态学变化明显,神经元数量明显减少.随着暴露剂量增加,仔鼠海马CaMKⅡ含量逐渐下降,C、D组与E组比较差异有统计学意义(P<0.05),A、B组与E组比较差异无统计学意义(P>0.05).实验组间比较,A组与D组差异有统计学意义(P<0.05),B、C组与D组差异无统计学意义(P>0.05).结论 高剂量母源性BDE-209暴露可以导致仔鼠海马组织结构发生改变,神经无数黾减少,CaMKⅡ含量下降,影响仔鼠神经系统的发育.

abstractsObjective To study the effect of maternal deca-brominated diphenyl ether (PBDE-209) exposure on the microstructure and calmodulin-dependent protein kinase Ⅱ (CaMK Ⅱ) content in the hippocampus of the offspring rats. Methods Three-month-old female Wistar rats were exposed to peanut oil suspensions of commercial BDE-209 administered by daily oral garage at the doses of 100, 300, 600 and 1200 mg/kg (groups A, B, C, and D, respectively). The control rats (group E) were given only peanut oil of the same volume. The microstructure of the hippocampus of 5 offspring rats from each group was observed microscopically using HE staining. Ten offspring rats from each group were examined for CaMK Ⅱ content in hippocampus using enzyme-linked immunosorbent assay. Results Obvious histomorphological changes were found in the hippocampus of the offspring rats in groups C and D. The CaMK Ⅱ content in the hippocampus decreased with the increase of the doses of BDE-209 exposure, and compared with group E, the offspring rats in groups C and D showed significantly decreased CaMK Ⅱ content in the hipposcampus (P<0.05, respectively), but those in groups A and B showed no significant variations in CaMK Ⅱ content (P>0.05, respectively). Among the experimental groups, compared with group D, the offspring rats in group A showed significant decreased in CaMK Ⅱ (P<0.05), but those in group B and C showed no significant variations (P>0.05). Conclusion Maternal BDE-209 exposure can induce histological changes in the hippocampus, decrease the neuronal number, and lower CaMK Ⅱ content in the hippoeampus of the offspring rats to affect the development of the nervous system.