摘要目的 探讨Notch受体阻断剂MW167对胶质瘤细胞系U87增殖及凋亡的影响.方法 采用四甲基偶氮唑蓝(MTT)法检测MW167对体外培养的胶质瘤细胞系U87的增殖抑制作用.流式细胞仪AnnexinV-FITC和PI双染检测凋亡率.结果 MTT法检测显示MW167能明显抑制U87细胞的增殖并呈剂量依赖关系:流式细胞仪检测显示MW167可诱导U87胶质瘤细胞发生凋亡并呈剂量依赖关系.结论 MW167明显抑制U87胶质瘤细胞的增殖并诱导其发生凋亡,提示Noah受体阻断剂MW167对人脑胶质瘤的治疗具有潜在的临床应用价值.

abstractsObjective To observe the effect of Notch receptor blocker MW167 on the proliferation and apoptosis of U87 glioma cell line in vitro. Methods The inhibitory effect of MW167 on the proliferation of U87 cells was investigated by MTT assay, and flow cytometry with Annexin-FITC and PI staining was performed to detect the apoptotic rate of U87 cells after MW167 treatment. Results MTT assay demonstrated an obvious concentration-dependent inhibitory effect of MW167 on the proliferation of U87 cells. Flow cytometry showed that MW167 also concentration-dependently induced U87 cell apoptosis. Conclusion MW167 significantly inhibits the proliferation and induces apoptosis of U87 glioma cells, suggesting the potential therapeutic value of MW167 in the treatment of human glioma. [Key wnrds] Notch signaling;MWI67;Proliferation;Apoptosis;Glioma