摘要目的 研究血红素氧化酶Ⅰ型(HO-1)在胶质瘤组织中的表达及意义.方法 (1)选择中山大学孙逸仙纪念医院神经外科自2010年1月至2015年1月采用手术治疗的胶质瘤患者56例,其中WHO分级Ⅰ级4例,Ⅱ级16例,Ⅲ级10例,Ⅳ级26例.将Ⅰ、Ⅱ级胶质瘤分为低级别胶质瘤组,将Ⅲ、Ⅳ级胶质瘤分为高级别胶质瘤组.免疫组化染色检测胶质瘤中HO-1的表达.(2)采用R语言survival工具包分析癌症基金组图谱(TCGA)数据库中1107例胶质瘤患者的预后与HO-1蛋白表达的关系.结果 (1)免疫组化染色结果显示高级别胶质瘤中HO-1高表达率(75%)高于低级别胶质瘤(30%),差异有统计学意义(P<0.05).高级别胶质瘤中HO-1的表达水平高于低级别胶质瘤,差异有统计学意义(P<0.05).用HO-1评分绘制ROC曲线,曲线下面积为0.747(P=0.002),说明HO-1的表达水平对胶质瘤分级的判断有参考价值.Log-rank检验显示HO-1低表达组患者的累积生存率高于HO-1高表达组,差异有统计学意义(P<0.05).(2)TCGA数据库中HO-1低表达组患者的同期生存率高于HO-1高表达组,差异有统计学意义(P<0.05).结论 胶质瘤中HO-1蛋白表达水平与胶质瘤恶性程度及预后相关,有望作为反映胶质瘤诊断及治疗的新生物学指标,并成为胶质瘤治疗和研究的新靶点.

abstractsObjective To investigate the expression and biological value of heme oxygenase I (HO-1) in gliomas. Methods Fifty-six patients with gliomas, admitted to and accepted surgery in our hospital from January 2010 to January 2015, were chosen in our study; WHO grade I was noted in 4 patients, grade Ⅱ in 16, grade Ⅲ in 10, and grade IV in 26 patients; patients with grade I and Ⅱ were as low-grade glioma group and patients with grade Ⅲ and IV were as high-grade glioma group. The HO-1 expression in the two groups was detected by immunohistochemistry. R-langrage survival tool was used to analyze the relation between HO-1 expression and prognosis of 1107 patients with gliomas selected from The Cancer Genome Atlas (TCGA) database. Results Significant differences of HO-1 expression were observed in different grades of gliomas (P<0.05), and HO-1 high-expression rate in the high-grade gliomas (75%) was significantly higher than that in the low-grade group (30%, P<0.05). HO-1 protein expression level in the high-grade gliomas was significantly higher than that in the low-grade group (P<0.05). Moreover, area under the curve (AUC) of the receiver operating characteristic curve was suggested that the HO-1 could be an ideal determine factor (AUC=0.747, P=0.002). Log rank analysis indicated that the accumulate survival rate in patients with low HO-1 expression was significantly higher than that in patients with high HO-1 expression (P<0.05). TCGA database analysis showed that simultaneous survival rate in patients with low HO-1 expression was significantly higher than that in patients with high HO-1 expression (P<0.05). Conclusion Expression of HO-1 is correlated with the malignant degrees and prognoses of gliomas, and it has potential to be a novel biological marker for the diagnosis and treatment of gliomas; furthermore, HO-1 could also be a target for the study and treatment of gliomas.