摘要目的 探讨碱性螺旋-环-螺旋转录因子少突胶质细胞因子1/2(Olig1、Olig2)在新生大鼠生后早期感染导致的脑损伤中的表达变化. 方法 将192只新生3 d (P3)SD大鼠按随机数字表法分为对照组、脂多糖(LPS)组、LPS+缺氧组,每组64只,其中后2组分别于P3、P6时腹腔注射LPS(0.25 mg/kg)各1次,对照组同法给予同剂量生理盐水,LPS+缺氧组在注射LPS前给予常压缺氧处理2h.依据取材时间点的不同将上述各组进一步分为P7、P10、P14、P21亚组,每亚组16只.采用Western blotting及免疫组化染色检测新生大鼠脑组织中Olig1、Olig2蛋白及阳性细胞的表达,采用HE染色观察新生大鼠脑组织病理变化. 结果 LPS组与LPS+缺氧组Olig1、Olig2蛋白表达水平及阳性细胞数均在P7时开始增加,在P10时达高峰,随后下降,且各时间点的表达水平及数量均明显高于对照组,差异均有统计学意义(P＜0.05);LPS+缺氧组各时间点Olig1、Olig2蛋白表达水平及阳性细胞数均明显高于LPS组,差异均有统计学意义(P＜0.05).HE染色显示LPS组与LPS+缺氧组神经细胞发生水肿,数目减少,核固缩、碎裂、偏移. 结论 新生大鼠生后早期感染可引起脑损伤,缺氧与感染双重因素同时存在会更进一步加重脑损伤,且该病理生理过程有Olig1、Olig2的参与.

abstractsObjective To explore the expressions of transcription factors Olig1 and Olig2 in neonatal rats with early infection associated with cerebral injury.Methods Sprague-Dawley rats (3-d old) were randomly divided into normal saline NS group,lipopolysaccharide LPS group,lipopolysaccharide and hypoxia LPS+H group.SD rats of LPS group and LPS+H group were intraperitoneally administered 0.25 mg/kg LPS at 3-d old (P3) and 6-d old (P6);rats of NS group were given normal saline by the same way and dose;rats of LPS+H group were performed atmospheric anoxic treatment for 2 h.Each group was divided into subgroups of 7-d old (P7),10-d old (P10),14-d old (P14) and 21-d old (P21) according to different times of sacrifile(n=16).HE staining was used to observe the pathological changes of brain tissues,Western blotting and immunohistochemical method were performed to detect the expressions of Olig1 and Olig2 in brain tissues.Results Expressions of Olig1 and Olig2 in the LPS group and LPS+H group began to increase on P7,peaked on P10,and then decreased,which were significantly higher than those in the NS group at any time point (P＜0.05);Expressions of Olig 1 and Olig2 in LPS+H group were statistically higher than those in LPS group at any time point (P＜0.05).Number of Olig1-and Olig2-positive cells in the LPS group and LPS+H group began to increase on P7,peaked on P10,and then decreased,which were significantly larger than that in NS group at any time point (P＜0.05);number of Olig1-and Olig2-positive cells in LPS+H group was significantly larger than that in LPS group at any time point (P＜0.05).Edema,reduced number,nuclear fixation,fragmentation and migration were noted in neurons of LPS group and LPS+H group by HE staining.Conclusion Postnatal early infections can cause brain damage in neonatal rats;hypoxia and infection aggravate the brain damage;Olig1 and Olig2 are involved in pathophysilogical process of cerebral injury.