摘要目的 探讨微小miRNA-134(miR-134)对新生儿缺氧缺血性脑病(HIE)的神经功能调控作用及机制. 方法 60只新生7dSD大鼠按随机数字表法分为缺氧缺血组、假手术组,每组30只,每组大鼠按照缺氧缺血后不同时间分为0h、1d、7d3个亚组.每个亚组10只.采用改良Rice法制作HIE模型,缺氧缺血后0h、1d、7d,实时荧光定量PCR检测2组大鼠脑组织miR-134表达量的变化,免疫组化染色检测2组大鼠脑组织人单丝氨酸蛋白激酶1 (Limk1)表达的变化.结果 与假手术组比较,缺氧缺血组大鼠各时间点脑组织中miR-134的表达均减少,差异有统计学意义(P＜0.05).缺氧缺血后0h、1d、7d缺氧缺血组大鼠脑组织中miR-134的表达依次减少,差异有统计学意义(P＜0.05);与假手术组比较,缺氧缺血组大鼠各时间点脑组织中Limk1的表达均增加,差异有统计学意义(P＜0.05).缺氧缺血后0h、1d、7d缺氧缺血组大鼠脑组织中Limk1的表达依次增加,差异有统计学意义(P＜0.05). 结论 miRNA-134可能通过负性调控Limk1的表达,对新生儿缺氧缺血性脑组织的神经重塑及神经系统的发育起重要作用.

abstractsObjective To explore the neuro-functional regulation and mechanism ofmiRNA in neonatal hypoxic ischemic encephalopathy (HIE).Methods Sixty 7-d-old SD rats were randomly divided into a sham-operated group and a hypoxic ischemic group (n=30);HIE models were induced by modified Rice method.Real-time fluorescent quantitative PCR was employed to detect the miR-134 expression and immumohistochemical staining was used to detect the Limk1 expression in the brain tissues of two groups 0,1,and 7 d after hypoxia ischemia (10 rats in each time point).Results As compared with sham-operated group,hypoxic ischemic group had significantly lower miR-134 level (P＜0.05);and 0,1,and 7 d after hypoxia ischemia,the miR-134 level decreased in sequence,with significant differences between each two time points (P＜0.05).As compared with sham-operated group,hypoxic ischemic group had significantly higher Limk1 level (P＜0.05);and 0,1,and 7 d after hypoxia ischemia,the Limk1 level increased in sequence,with significant differences between each two time points (P＜0.05).Conclusion MiRNA-134 may play an important role in neuronal plasticity and development of nervous system in brain tissues of HIE by regulating Limk1 expression.