摘要目的 了解水蛭素对人增生性瘢痕Fb(HSFb)功能的影响.方法 体外培养HSFb,在DMEM培养液中分别加入1、10、50 kU/L水蛭素溶液,设定为相应浓度组,每组9孔;设常规培养为对照组.于培养后24、48、72 h检测各组细胞抑制率,测定细胞分泌TGF-β1水平,以及细胞Ⅰ、Ⅲ型前胶原mRNA表达. 结果 10 kU/L水蛭索作用24、48、72 h后,HSFb的生长抑制率分别为(29.3±0.9)%、(30.1±0.3)%、(45.2±1.9)%,均高于对照组[(0.0±0.0)%,P<0.05];其他浓度组部分时相点与对照组比较,差异有统计学意义(P<0.05).水蛭素作用于HSFb 48 h后,1、10、50 kU/L水蛭素组TGF-β1含量分别为(228.5±1.8)、(210.5±11.1)、(168.5±14.1)pg/mL,与对照组(265.0±1.5)pg/mL比较,后2组差异有统计学意义(P<0.05).10、50 kU/L的水蛭素可抑制HSFb Ⅰ、Ⅲ型前胶原mRNA表达. 结论 10、50 kU/L水蛭素溶液对HSFb增殖以及TGF-β1和胶原的分泌具有一定抑制作用.

abstractsObjective To study the effect of hirudin on the function of human hyperplastic scar fi-broblasts (HSFBs). Methods HSFBs were cultured in vitro. Hirudin solution in the concentration of 1, 10, and 50 kU/L was respectively added into DMEM culture medium to form 1, 10, and 50 kU/L hirudin groups, with 9 wells in each group. HSFBs cultured without hirudin were set up as control group. Cell inhi-bition rate, secretion level of TGF-β1 from cells, and expression levels of mRNA of type Ⅰ and Ⅲ precolla-gem were determined at 24, 48, and 72 h after culture. Results Inhibition rates of HSFBs growth was re-spectively ( 29.3 ± 0.9) % , ( 30.1 ± 0.3 ) % , and (45.2 ± 1.9 ) % when cultured with 10 kU/L hirudin for 24, 48, and 72 hs, which were higher than those in control group [ (0.0±0.0)% , P <0.05]. There was statistically significant difference between control group and 1 and 50 kU/L hirudin groups in the inhibition rates of HSFBs at some time points ( P <0.05). Secretion level of TGF-β1 of HSFBs in 1, 10, 50 kU/L hirudin groups was respectively (228.5±1.8), (210.5±11. 1), and (168.5±14.1) pg/mL when cul-tured for 48 hs, of which the last 2 figures were significantly lower than that of control group [ (265.0±1.5) pg/mL, P < 0.05 ]. Hirudin in the concentration of 10 and 50 kU/L could inhibit the expression of mRNA of type Ⅰ and Ⅲ precollagen in HSFBs. Conclusions Hirudin solution in the concentration of 10 and 50 kU/L can inhibit the proliferation of HSFBs and secretion of TGF-β1 and collagen in certain degree.