摘要目的 研究miR-124和miR-138参与调控健侧颈7移位术后脑功能重塑的作用.方法 取雄性SD大鼠126只,根据造模和手术处理方式分为手术组、单纯撕脱组和假手术组.手术组制备右侧全臂丛神经根性撕脱伤模型,切取左侧颈7神经根全根作为供体通过尺神经桥接移位修复右侧正中神经;单纯撕脱组仅制备右侧建立全臂丛神经根性撕脱伤模型,左侧臂丛神经仅暴露;假手术组仅暴露双侧臂丛神经.采用RT-PCR法检测不同组大鼠术后不同时间点双侧大脑运动皮层miR-124和miR-138的表达情况,并进行相关性分析.结果 手术组和单纯撕脱组miR-124和miR-138的表达随时间呈现动态变化,而假手术组各时间段并无明显差异.miR-124和miR-138表达量,手术组高于单纯撕脱组,单纯撕脱组高于假手术组,差异均具有统计学意义(P<0.05).手术组和假手术组术后各时间点左右侧大脑半球miR-124和miR-138表达量差异无统计学意义(P>0.05).单纯撕脱组左侧大脑半球miR-124和miR-138表达水平显著高于右侧(P<0.05).结论 miR-124和miR-138可能参与了健侧颈7移位术后脑功能重塑的调节过程,从而调控神经修复术的效果.

abstractsObjective To investigate the role of miR-124 and miR-138 in regulating the brain trans-hemispheric functional reorganization after the contralateral C7 transfer. Methods According to the model establishment and surgical treatment, 126 male SD rats were divided randomly into three groups: operation group, simple avulsion group and sham group. In the operation group, the right total brachial plexus avulsion model was made and the left C7 nerve root was cut as a donor to repair the right median nerve through ulnar nerve bridging. In the simple avulsion group, only the right total brachial plexus avulsion model was made and the left brachial plexus was only exposed. The sham group only exposed the bilateral brachial plexus. RT-PCR was used to detect the expression of miR-124 and miR-138 in bilateral brain motor cortex of rats at different time points after operation, and the correlation was analyzed. Results The expression level of miR-124 and miR-138 in the operation group and simple avulsion group showed dynamic changes with time, while in the sham group they showed no significant difference between different time points. The expression level of miR-124 and miR-138 in the operation group were greater than those in the simple avulsion group, and those in the simple avulsion group was higher than those in the sham operation group (P<0.05). There was no significant difference in the expression of miR-124 and miR-138 of the operation group and the sham operation at each point after operation between brain left and right hemispheres (P>0.05). The expression levels of miR-124 and miR-138 in the left hemisphere of the simple avulsion group were significantly higher than those in the right hemisphere (P<0.05). Conclusion The miR-124 and miR-138 may be invovled in the regulation of brain function remodeling after the contralateral C7 nerve transfer.