摘要目的 探讨复方当归注射液对人成纤维细胞增殖的抑制作用及机制.方法 分别给予人成纤维细胞10、20、40、80 mg/ml浓度的复方当归注射液,作用48、72 h后利用MTS方法检测其增殖水平变化,并通过ELISA检测了解TGF-β1表达水平,采用实时定量荧光PCR (real-timePCR)检测复方当归注射液作用后TGF-β1的变化水平.结果 MTS检测结果显示复方当归注射液作用后,20、40、80 mg/ml组的细胞增殖抑制(P＜0.05);real-time PCR检测结果显示复方当归注射液作用48 h后,成纤维细胞TGF-β1的转录水平与对照组相比,10、20、40 mg/ml组均降低(P＜0.05);ELISA检测结果表明复方当归注射液作用48 h后,随着药物浓度的增加,TGF-β1表达水平受到抑制(P＜0.05).结论 复方当归注射液能够抑制人成纤维细胞TGF-β1的表达水平,提示其可以抑制人成纤维细胞增殖,并且在作用一定时间后抑制水平呈浓度依赖性,从而可能影响肌腱粘连的发生.

abstractsObjective To investigate the inhibitory effect and mechanism of compound angelica injection on the proliferation of human fibroblasts.Methods Compound angelica injection with 10,20,40 and 80 mg/ml concentrations was administered on human fibroblasts respectively.MTS method was used to detect the proliferation level of human fibroblasts after 48 and 72 hours.The expression level of TGF-β1 was detected by ELISA.Real-time PCR was used to detect the change level of TGF-β1 after compound angelica injection.Results MTS results showed that the proliferation of fibroblasts was inhibited in 20,40,80 mg/ml group (P＜0.05);real-time PCR results showed that the transcription level of TGF-β1 in fibroblasts decreased in 10,20,40 mg/ml group compared with the control group 48 hours after the treatment of compound angelica injection (P＜0.05).ELISA results showed that the expression of TGF-β1was inhibited with the increase of drug concentration 48 hours after the treatment of compound angelica injection (P＜0.05).Conclusion Compound angelica injection can inhibit the expression of TGF-β1 in human fibroblasts,suggesting that it can inhibit the proliferation of human fibroblasts,and the inhibition level is concentration-dependent after a certain period of time,which may affect the occurrence of tendon adhesion.