摘要目的:探讨miR-152对雪旺细胞(Schwann cells，SCs)迁移作用的影响。方法:构建大鼠坐骨神经损伤模型，分别于损伤后1、4、7、14 d采用qRT-PCR检测miR-152和CAND1基因在损伤神经中的表达情况；Western blot和qRT-PCR检测miR-152和CAND1在SCs中的表达；Transwell法测定SCs迁移能力；以荧光素酶报告基因和RNA免疫沉淀法(RNA binding protein immunoprecipitation，RIP)证实miR-152与CAND1的相关性。结果:损伤神经近端和远端神经组织中miR-152水平显著升高，miR-152的过度表达促进了SCs迁移，而miR-152抑制剂转染的SCs则明显抑制了细胞迁移。结果提示CAND1是miR-152的靶基因，此外miR-152对CAND1的表达具有负调控作用，CAND1表达上调阻断了miR-152介导的促进SCs迁移作用。结论:周围神经损伤后，miR-152可以通过抑制CAND1的表达促进SCs迁移作用。

abstractsObjective:To investigate the effect of miR-152 on the migration of Schwann cells (SCs).Methods:The model of sciatic nerve injury was established in rats. The expression of miR-152 and cullin-associated and neddylation-dissociated 1 (CAND1) in injured nerve was detected by qRT-PCR at 1, 4, 7 and 14 days after injury; the expression of miR-152 and CAND1 in SCs was detected by Western blot and qRT-PCR; the migration ability of SCs was determined by Transwell assay; the correlation between miR-152 and CAND1 were verified by Luciferase reporter gene and RNA immunoprecipitation (RIP) assay.Results:In the current study, firstly, on the 1st, 4th, 7th and 14th day after nerve injury, the levels of miR-152 in proximal and distal nerve segments increased significantly. Moreover, overexpression of miR-152 led to the promotion of SCs migration, while a remarkably repression of migration was observed in the cell which were transfected with miR-152 inhibitor. The results suggest that CAND1 was the target gene of miR-152. In addition, miR-152 negatively regulates the expression of CAND1. The up regulation of CAND1 expression blocks miR-152 mediated promotion of SCs migration.Conclusion:miR-152 can promote SCs migration by inhibiting the expression of CAND1 after peripheral nerve injury.