摘要目的 了解青海同德藏族人群乙型肝炎病毒(HBV)基因型、血清型的分布状况.方法 用巢式PCR扩增青海同德藏族人群表面抗原(HBsAg)阳性者的HBV S、C基因,测定其序列,判定其基因型和血清型.结果 源于自然人群的311份血清标本中,同时获得S和C基因序列者271份,其中C型基因10例,占3.7%,C/D重组型261例,占96.3%;血清ayw2亚型259例,占95.6%,adr亚型10例,占3.7%,adw2亚型2例,占0.7%.结论 青海同德藏族人群感染HBV以C/D重组型为主,血清型以ayw2为主.

abstractsObjective To explore the distribution of HBV genotype and serotype from Tibetan in Tongde, Qinghai. Methods Nested polymerase chain reaction (nPCR) was used for amplification of S gene and C gene of HBV from sera carried by Tibetan chronic HBV carrier in Tongde, Qinghai, then the HBV DNA positive products were sequenced by direct sequencing. Genotype and serotype were identified by analysis of sequence result. Results 271, which come from 311 sera samples with positive H BsAg randomly selected from natural community, were amplified and sequenced in beth S gene and C gene successfully, 10(3.7 % ), 261 (96.3 % ) out of them were identified as genotype C, recombinant between genotypes C and D respectively; 259 (95.6%), 10 (3.7 % ), 2 (0.7%) belonged to serotype ayw2,adr,adw2 respectively. Conclusion The recombinant between genotypes C and D was the main genotype in Tibetan chronic carrier with hepatitis B in Tongde, Qinghai;the serotype of this areas was consisted largely of ayw2.