摘要目的 探讨慢性乙型肝炎患儿HBV基因型与乙肝病毒大蛋白的关系.方法 采用实时荧光PCR法和ELISA法分别检测138例处于乙肝病毒活动期的慢性乙型肝炎患儿血清中的HBV DNA和乙肝病毒大蛋白并鉴定其基因型.结果 乙肝病毒大蛋白吸光度与HBV DNA载量存在正相关(r=0.85,P＜0.05);HBV基因B型与HBV基因C型的ALT水平、乙肝病毒大蛋白吸光度和HBVDNA载量差异无统计学意义(P＞0.05,P＞0.05,P＞0.05).结论 乙肝病毒大蛋白水平与HBVDNA载量具有良好的正相关性,表明乙肝病毒活动期的慢性乙肝患者体内乙肝病毒大蛋白与病毒复制程度密切相关,乙肝病毒基因型与乙肝病毒大蛋白无关.

abstractsObjective To study the relationship between hepatitis B virus genotypes and hepatitis B virus large envelope protein. Methods A total of 138 surveyed children with chronic active HBV infection were enrolled real-time fluorescence PCR method was employed to quantify serum HBV DNA levels and classify the HBV genotypes. Hepatitis B virus large envelope protein was detected by using enzyme linked immuno sorbent assay (ELISA). Results The absorbance of hepatitis B virus large envelope protein was positively correlated with hepatitis B virus DNA levels (r=0.85, P＜0.05); Serum ALT levers, the absorbance of hepatitis B virus large envelope protein and hepatitis B virus DNA levels (P＞0.05,P＞0.05,P＞0.05), were no significant difference between hepatitis B virus genotype B and hepatitis B virus genotype C. Conclusion There was a close correlation between the absorbance of hepatitis B virus large envelope protein and HBV DNA levels. Hepatitis B virus large envelope protein was a reliable serological marker that can reflect the replication of hepatitis B virus and there was no relationship between hepatitis B virus genotypes and Hepatitis B virus large envelope protein.