摘要目的 比较DC-LMP2与rAd-LMP2在BALB/c小鼠中的免疫效果.方法 从BALB/c小鼠中分离骨髓细胞,在细胞因子作用下体外诱导培养获得小鼠树突状细胞(mDC),再以100 MOI的含EB病潜伏膜蛋白2的重组腺病毒(rAd-LMP2)感染,获得DC-LMP2,免疫酶法确定LMP2在DC中表达.于0,2,4周,分别以PBS、DC-LMP2和rAd-LMP2肌肉免疫BALB/c小鼠,第5和8周检测小鼠体内LMP2特异性细胞应答水平.结果 骨髓细胞体外成功诱导获得DC,LMP2在DC中表达.DC-LMP2和rAd-LMP2均可诱导LMP2特异性细胞免疫应答,随时间推移而减弱.rAd-LMP2诱导特异性细胞免疫应答水平高于DC-LMP2.结论 肌肉免疫rAd-LMP2能够在BALB/c小鼠中诱导较强的LMP2特异性细胞免疫应答.

abstractsObjective To compare immunological effect induced with DC-LMP2 and rAd-LMP2 in BALB/c mice.Mehtods Bone marrow cells were separated from BALB/c mice,and induced into murine dendritic cells (mDCs) cultured by RPMI 1640 medium supplemented with 10％ fetal calf serum and recombinant murine cytokines in vitro.mDCs were infected by recombinant adenovirus including latent membrane protein Ⅱ (LMP2) gene (rAd-LMP'2) of Epstein-Barr virus(EBV) at a dose of 100 multiplicity of infection (MOI),and matured mDC-LMP2 was harvested at 48 hours later.The LMP2 expression in mDCs was detected by immunoenzyme.BALB/c mice were immunized with PBS DC-LMP2 and rAd-LMP2 by intramuscular injection at 0,2,4 weeks.LMP2 specific cytotoxic lymphocytes (CTLs) in BALB/c mice were detected at 5th week and 8th week with enzyme-linked immunosorbent spot (ELISPOT).Results mDCs from bone marrow cells were harvested.LMP2 was detected in DC-LMP2.LMP2 specific CTLs were stimulated by both DC-LMP2 and rAd-LMP2 and decreased with time elapsed.LMP2 specific CTLs stimulated by rAd-LMP2 were higher than that by DC-LMP2 in BALB/c mice.Conclusion Immunization with rAd-LMP2 by intramuscular injection was more fitful than that with DC-LMP2 in BALB/c mice.