摘要目的 比较评价2种登革病毒NS1抗原检测试剂和1种核酸检测试剂在登革热早期诊断中的应用,为登革热实验室检测试剂选择提供依据.方法 选取登革病毒感染发病早期患者血清278份,非登革热患者血清100份,对3种检测试剂进行比较评价.结果 发病5d内患者血清样本,PCR-荧光探针法敏感性为98.9％ (180/182),ELISA法为97.8％ (178/182),胶体金法为81.9％(149/182),发病后6～7d的血清样本,敏感性均有所下降,NS1 ELISA法相对恒定,三种检测试剂均具有较高的特异性.结论 实时荧光RT-PCR法核酸检测试剂在发病早期敏感性最高,ELISA法检测NS1抗原法与核酸检测一致性最强,胶体金法敏感性略差,但更简便快捷,适合基层和现场使用,NS1抗原检测可用于我国登革热病例的监测与检测.

abstractsObjective To evaluate the performance of two commercially available dengue nonstructural 1 (NS1) antigen assays and a reverse-transcription polymerase chain reaction (RT-PCR) for the diagnosis of dengue.Methods A panel of dengue positive and negative sera was assembled,and 278 sera from suspected Dengue patients and 100 sera from non-dengue infected people were included to evaluate the diagnostics.Results The analysis showed RT-PCR to be the most sensitive and specific (100％) diagnostic method during the first 5 days of fever,The overall sensitivity of dengue NS1 antigen assays within the same period was 97.8％ (178/182)for ELISA and 81.9％ (149/182)for NS1 Cassette.And all the 3 diagnostics were highly specificity.Conclusion Real time RT-PCR is the most sensitive method at early stage of the illness; NS1 antigen detection is a convenient method for the diagnosis of dengue fever in a primary healthcare setting,which can be used in the early detection of dengue and hence could play an important role in routine surveillance efforts to control dengue outbreaks in China.