摘要目的 检测潮汕地区食管癌患者血清中人乳头瘤病毒(HPV)相关抗体的存在情况,为我国食管癌患者的监测提供参考数据.方法 分别构建包含HPV16型E6/E7和HPV18型E6/E7基因的pSecTag重组载体,转染293细胞后分别表达HPV16型E6/E7和HPV18型E6/E7的融合蛋白,以融合蛋白为抗原,用免疫酶法检测76份食管癌患者血清及149份正常体检人群血清中HPV的IgG抗体;用L1做抗原的ELISA法检测相同样本的血清.结果 使用免疫酶法,E6/E7融合蛋白做抗原在所有血清标本中只有一份食管癌患者血清显示免疫阳性;使用ELISA法,L1做抗原,76例食管癌患者中有37例检测到抗体IgG阳性反应,阳性率为48.7％,149例体检人群中,79例有抗体IgG阳性反应,阳性率为53.0％.经统计分析二者无显著性差异.结论 HPV相关抗体检测方法尚不能作为食管癌的筛查工具,HPV感染的血清学检测用于食管癌的诊断有待于进一步的研究.

abstractsObjective To detect the presence of HPV associated antibodies in the serum of patients diagnosed with esophageal cancer in Chaoshan region and to provide data that could possibly be used as reference for the monitoring of patients with esophageal cancer.Methods The pSecTag recombinant vectors containing the HPV16 E6/E7 and HPV18 E6/E7 genes were constructed respectively to express HPV16 E6/ E7 and HPV18 E6/E7 fusion proteins in 293 cell line after transfecting the cell line.Immunoenzymatic method was employed with the fusion proteins as antigen to detect IgG antibody against HPV in serum of 76 esophageal cancer patients and 149 normal persons undergoing health checkup.In addition,the same sera were assessed by enzyme-linked immunosorbent assay (ELISA) method using L1 as antigen.Results Only one esophageal cancer patient's serum presented as positive for IgG by immunoenzyme technique when E6/ E7 was used as antigen.However,when the L1 was used as antigen in ELISA assay,37 of the 76 cases of esophageal cancer patients (48.7％) were HPV antibody-positive.Among the 149 health checkup persons,79 (53.0％) were HPV antibody-positive.No significant difference was found between the two groups (P ＞ 0.05).Conclusion HPV related antibodies in the serum remain inapplicable as a screening tool for serological detection of esophageal cancer.Further research is needed to understand the role and diagnostic applications of HPV infection in esophageal cancer.