摘要目的:通过mariPOC ?多重分析法与直接免疫荧光法(direct immunofluoresence assay，DFA)检测肺炎患儿中常见的呼吸道病毒，对检测结果进行比较来评估mariPOC ?法的检测性能。 方法:采集肺炎患儿的鼻咽拭子标本，用mariPOC ?多重分析法和DFA检测甲型流感病毒、乙型流感病毒、呼吸道合胞病毒、人偏肺病毒、副流感病毒1、2、3型和腺病毒，比较两种方法的检出率和检测结果的一致性。同时用实时荧光PCR法(real-time PCR)为参比方法对两种方法检测不一致的结果进行验证。 结果:本研究共纳入400名肺炎住院患儿，mariPOC ?多重分析法和DFA法总阳性率分别为40.25%、38.25%。mariPOC ?多重分析法与DFA法的阳性一致率为92.16%，阴性一致率为91.90%，总一致率为92.00%。其中有32例不一致结果，用实时荧光PCR法进行确认，20个结果与mariPOC ?法一致，12个与DFA法一致。mariPOC?多重分析法与real-time PCR相比较，敏感度为93.06%，特异度为100.00%，总符合率达97.00%。 结论:mariPOC ?法与DFA镜检法相比，在肺炎患儿呼吸道病毒检测中的检测性能良好，与real-time PCR相比敏感度、特异度和总符合率良好，mariPOC ?多重分析法具有敏感性高、检测快速、智能等明显优势，可有效应用于临床检验。

abstractsObjective:mariPOC ? multianalyte assays and direct immunofluorescence assays (DFA) were used to detect respiratory viruses in children with pneumonia and evaluate the performance of mariPOC ? method by comparing the results. Methods:Nasopharyngeal swab samples were tested by both mariPOC ? and DFA for influenza A and B, respiratory syncytial viruse, adenoviruse, and parainfluenza 1, 2, and 3 viruses. Real-time PCR was also used to detect the DNA of FluA、FluB、RSV、PIV 1、2、3 and ADV as reference method, in order to verify the inconsistent result of the above two method. Results:Four hundred children with pneumonia were enrolled. The total positive rates of mariPOC ? and DFA were 40.25% and 38.25% respectively. According to the results, the positive consistency between mariPOC ? and DFA was 92.16% and the negative consistency was 91.90%, the overall agreement rate was 92.00%. There were 32 samples where mariPOC ? and DFA gave contradicting results. In real-time PCR confirmation, the result of 20 samples were consistent with mariPOC ? test and 12 agreed with DFA. Compared with real-time PCR, the sensitivity of mariPOC ? was 93.06%, the specificity was 100.00%, and the total coincidence rate was 97.00%. Conclusions:mariPOC ? multianalyte assays had good correlation with DFA for detection of respiratory viruses in children with pneumonia. Compared with the reference method real-time PCR, it has better sensitivity, specificity and total coincidence rate. mariPOC ? multianalyte assay has the obvious advantages of high sensitivity, fast detection and intelligence, which can be effectively applied to clinical examinations.