摘要目的:研究微波对模拟感染性废物中人腺病毒2型(human adenovirus, HAdV-2)的灭活作用，并探讨其分子机制。方法:将25 μl HAdV-2病毒悬液分别滴染医用一次性手套、口罩、棉签模拟感染性废物，在不同微波条件下对其进行辐照：手套和口罩在300 W、500 W、700 W分别辐照30 s、60 s、90 s，棉签在500 W、700 W分别辐照60 s、90 s、120 s，记录温度变化，50%终点法计算灭活对数值评价微波灭活效果，qPCR检测病毒增殖能力，PCR检测病毒 Penton、 Fiber、 Hexon、 E2 B基因损伤情况。单独以微波辐照过程中的最高温度76 ℃处理病毒，研究微波消毒过程是否存在非热效应。 结果:微波辐照感染性废物后，口罩、手套载体温度快速升高，最高温度76 ℃；棉签载体升温较慢，最高温度65 ℃。微波对HAdV-2的灭活效果与微波功率、辐照时间成正相关。口罩、手套组微波700 W、60 s，灭活对数值可达3.0；棉签组微波700 W、120 s，灭活对数值仍小于3.0，表明不同载体中微波灭活病毒的条件存在差异。qPCR结果显示，微波能使病毒的增殖能力减弱。，微波辐照对病毒 Penton、 Fiber基因无影响，但对 Hexon、 E2 B基因产生一定损伤。单独热处理对HAdV-2的灭活效果弱于微波辐照，且对 Hexon、 Penton、 Fiber、 E2 B基因无损伤，表明微波灭活过程中存在非热效应。 结论:微波辐照可以通过热效应以及非热效应对模拟感染性废物中的HAdV-2产生灭活作用，其对病毒DNA损伤是病毒灭活的机制之一。

abstractsObjective:To study the inactivation effects of microwave on human adenovirus 2 (HAdV-2) in simulated infectied wastes, and to explore its molecular mechanism.Methods:25 μl of HAdV-2 virus suspension was dripped with medical disposable gloves, masks and cotton swabs to simulate infectied wastes, and irradiated under different microwave conditions: gloves and masks were irradiated for 30 s, 60 s, and 90 s at 300 W, 500 W, and 700 W, respectively. Cotton swabs irradiate 60 s, 90 s, 120 s at 500 W and 700 W respectively. Temperature changes were recorded, and the inactivation logarithmic values were calculated by the 50% endpoint method to evaluate the microwave inactivation effects, and the proliferation ability of the virus was detected by qPCR. The damage of Penton, Fiber, Hexon and E2 B genes was detected by PCR. The virus was treated with the highest temperature of 76 ℃ during microwave irradiation to study whether there was non-thermal effect during microwave disinfection. Results:After microwave irradiation of infectied waste, the temperature of masks and gloves carriers rises rapidly, with the highest temperature of 76 ℃. The temperature of the cotton swab carriers rose slowly, and the highest temperature is 65 ℃. The inactivation effect of microwave on HAdV-2 was positively correlated with microwave power and irradiation time. In the mask and glove group, microwave power of 700 W irradiated for 60 seconds, and the inactivation logarithm value could reach 3.0, In the cotton swab group, microwave power of 700 W irradiated for 120 seconds, and the inactivation logarithm value was still less than 3.0. This indicated that there were differences in the conditions for microwave inactivation of the virus in different carriers. The qPCR result showed that microwave irradiation could weak the proliferation ability of the virus. Microwave irradiation had no effect on the virus's Penton and Fiber genes, but caused some damage to the Hexon and E2 B genes. The inactivation effect of individual heat treatment on HAdV-2 was weaker than that of microwave irradiation, and there was no damage to the Hexon, Penton, Fiber, and E2 B genes. This indicated the presence of non thermal effects during the microwave inactivation process. Conclusions:Microwave irradiation can inactivate HAdV-2 in simulated infectied wastes through thermal and non-thermal effects, and its damage to viral DNA is one of the mechanisms of virus inactivation.