摘要目的:调查甘肃省东南地区蚊传虫媒病毒的种类与分布。方法:2023年在甘肃省陇南市和天水市部分地区使用诱蚊灯法采集蚊虫。根据形态学进行分类后约50只为1批。使用RT-qPCR法对采集的蚊虫标本进行乙型脑炎病毒、版纳病毒、盖塔病毒、库蚊黄病毒和塔黑纳病毒核酸检测。随后对阳性样本进行基因组测序，并使用生物信息学软件对病毒基因组进行遗传进化分析。结果:2023年6～8月在陇南市徽县、成县、武都区和天水市麦积区、秦州区共采集蚊虫4属9种8 176只，其中三带喙库蚊数量最多，占采集蚊虫总数的55.80%(4 562/8 176)，其次为淡色库蚊，占采集蚊虫总数的16.43%(1 343/8 176)。共获得263批标本，RT-qPCR检测显示1批标本乙型脑炎病毒阳性、2批标本版纳病毒阳性、3批标本盖塔病毒阳性和25批标本库蚊黄病毒阳性。结论:甘肃省东南地区的优势蚊种为三带喙库蚊，且当地蚊虫中携带多种虫媒病毒。

abstractsObjective:To investigate the species and distribution of mosquito-borne arboviruses in the southeastern part of Gansu province.Methods:In 2023, mosquitoes were collected using ultraviolet lights in Longnan and Tianshui regions of Gansu province. After classification by morphology, about 50 are only 1 batch. RT-qPCR was used to detect the RNA of Japanese encephalitis virus (JEV), Banna virus (BAV), Getah virus (GETV), Culex flavivirus (CxFV) and Tahyna virus (TAHV). The genomes of the positive samples were sequenced and the genetic evolution of the virus genomes were analyzed by bioinformatics software.Results:In total, there were 8 176 mosquitoes from 4 genera and 9 species collected from Longnan and Tianshui from June to August 2023. Culex tritaeniorhynchus was the most common species with 55.80% (4 562/8 176) of the total mosquitoes collected, followed by 16.43% (1 343/8 176) of Culex pipiens pallens. A total of 263 batches of samples were obtained, and the nucleic acid test showed that 1 batch was positive for JEV, 2 batches were positive for BAV, 3 batches were positive for GETV and 25 batches were positive for CxFV. Conclusions:Culex tritaeniorhynchus was the dominant mosquito species in the southeastern part of Gansu province, and local mosquitoes carried a variety of arboviruses.