摘要目的 对骨组织工程支架材料多孔碳酸钙陶瓷(PCCC)进行Ames试验以评价其潜在致突变性.方法 采用浸提法制备多孔碳酸钙陶瓷(PCCC)生理盐水浸提液.采用标准平板掺入法,计数TA97、TA98、TA100、TA102在4个不同浓度浸提液下,37℃培养48 h后的回复菌落数,测试其对鼠伤寒沙门菌的致突变比值(MR=实验组回变菌落数Rt/阴性对照组回变菌落数Rc).结果 阳性对照组各菌株回变菌落平均数均超过其相应阴性对照组回变菌落平均数的二倍以上(MRp＞2),多孔碳酸钙陶瓷(PCCC)浸提液各剂量组对鼠伤寒沙门菌的致突变比值MR均小于2.结论 多孔碳酸钙陶瓷(PCCC)不会引起鼠伤寒沙门菌的回复突变数增加,提示其无潜在致突变性.

abstractsObjective To study the mutagenicity of porous calcium carbonate ceramics as bone tissue engineering scaffold by Ames test.Methods Immerse PCCC into physiological saline to acquire PCCC solution.In the plate incorporation test of Salmonella typhimurium,the average number of spontaneous revertants of TA97,TA98,TA100,TA102 under four concentrations was calculated after incubation at 37℃ for 48 hours.We get the mutagenesis rates of murine typhoid Salmonella(MR).Results The mutagenesis rates of murine typhoid Salmonella in the experimental groups of all dosage levers are less than 2while the MR of the positive control groups are more than 2.Conclusion The PCCC will not cause the increase of the back mutation in murine typhoid Salmonella which shows that the material has no mutagenicity.