摘要目的 探讨神经因子诱导的克隆B(NGFI-B)基因和Nut相关因子1(Nurr1)基因在醛固酮瘤中的表达及意义.方法 应用实时逆转录-聚合酶链反应(Real time RT-PCR)检测34例醛固酮瘤和13例正常肾上腺组织中神经因子诱导的克隆B基因,Nur相关因子1基因,醛固酮合成酶基因(CYP11B2)和皮质醇合成酶基因(CYP11B1)的mRNA表达水平.结果 醛固酮腺瘤组织中NGFI-B基因的mRNA表达量与正常肾上腺组织的差异无统计学意义(P>0.05),醛固酮腺瘤组织中Nurr1基因呈高表达,与正常肾上腺组织的差异有统计学意义(P<0.01),醛固酮腺瘤组织中Nurr1基因的表达量与其CYP11B1和CYP11β2的表达量之间明显相关(分别r=0.434,P<0.01;r=0.376.P<0.05).结论 孤核受体Nurr1对醛固酮瘤组织中CYP11B2的表达有促进作用,醛固酮瘤组织CYP11B1和CYP11B2的表达是多种转录因子共同作用的结果.

abstractsObjective To investigate the expression and significance of NGFI-B and Nurr1 gene in aldosterone-producing adenomas (APA). Methods By using real-time RT-PCR assay, the expression of NGFI-B gene, Narr1 gene, CYP11B1 and CYP11 B2 was detected in a series of adrenal tissues, including 13 normal adrenals and 34 APA from patients with primary aldosteronism. Results There was no significant difference in the expression of NGFI-B gene between APA and normal adrenal (P>0.05). The expression of Nurr1 gene was increased in APA as compared with normal adrenal (P<0.01). In APA the expression of Nurr1 gene was correlated with that of CYP11B1 and CYPIlB2 (r=0.434,P<0.01 ;r= 0.376,P<0.05). Conclusion In APA Nurr1 can promote the expression of CYP11 B2 gene. Several transcription factors are involved in the expression of NGFIB genes.