摘要目的 观察重组人骨形态发生蛋白-2/异体骨复合骨、自体骨与异体骨分别用于兔腰椎融合后,不同时间点融合骨组织微结构的变化.方法 成年雄性新西兰大白兔45只,随机分为3组,每组15只.在每只兔的L5、L6横突间行腰椎后路植骨融合术,各组分别植入复合骨条,自体髂骨条以及单纯异体髂骨条,每组于术后第3、4、5周各处死5只大白兔,分离保存融合节段标本.用显微cT扫描后行骨组织定量分析.结果 术后3个时间点中,复合骨组和自体骨组新生骨小梁的强度和形态均要优于异体骨组且差异有统计学意义(P＜0.05).第3周,复合骨组的组织骨密度(TMD)为(433.98±2.64)mg/cm3,高于自体骨组(424.81±4.69)mg/cm3(P＜0.05);第4周,复合骨组的骨小梁厚度(Tb.Th)为(0.097±0.004)mm,高于自体骨组(0.082±0.003)mm(P＜0.01);第5周,复合组的组织矿含量(TMC)为(7.70±0.30)mg,高于自体骨组(7.00±0.24)mg(P＜0.01).结论 在兔腰椎后路横突间植骨融合中,重组人骨形态发生蛋-2/异体骨复合骨的成骨效应不低于自体骨,优于异体骨.

abstractsObjective To investigate the microstructure of fusion masses taken from the rabbits undergoing posterolateral lumbar spinal fusion with rhBMP-2/aUograft composite,or autograft and allograft.Methods Forty-five aduh male New Zealand white rabbits were randomly divided into three groups:rhBMP-2/allograft composite group,autograft group,and allograft group(n=15 in each group).The rabbits were subjected to L5 and L6 intertransverse process fusion using rhBMP-2/allograft composite,autogenous iliac crest bone graft,and allograft.Every 5 rabbits from each group were sacrificed at the 3th,4th,and 5th week after operation,and the fusion lnasse8 were harvested.A high resolution microCT was used to identify the densimetric and microstructural properties of tlle fusion masses.Results Compared with the allograft group at the three time-points,there was significant difference in all parameters in the trabecular bone of the rhBMP-2/allograft composite group and autograft group.At the 3th week,the mean value of tissue mineral density(TMD)in the composite group was(433.98±2.64)mg/cm3,which was significantly higher than that of autograft group(424.81±4.69)mg/cm3.At the 4th week.the mean value of trabecular thickness(Tb.Th)in the composite group was(0.097±0.004)mm,which was significantly higher than that of autograft group(0.082±0.003)mm.At the 5th week,the mean value of tissue mineral content (TMC)in the composite group was(7.70±0.30)mg,which was significantly higher than that of autograft group(7.00±0.24)mg.Conclusion The rhBMP-2/allograft composite was not inferior to autograft,but superior to allograft in accelerating and enhancing bone formation in a rabbit posterolateral lumbar spinal fusion model.