摘要目的 观察肿瘤坏死因子(TNF)联合肝脏肿瘤动脉灌注化疗栓塞术(TACE)治疗肝脏肿瘤的疗效以及对外周TNF-β的mRNA的表达影响.方法 将肝细胞癌(HCC)患者分为2组,单纯TACE治疗者48例为对照组,联合重组人TNF治疗者30例为实验组.通过肝脏增强CT测量比较肿瘤体积大小.应用逆转录-聚合酶链反应(RT-PCR)检测血液中TNF-β的mRNA的表达,半定量比较两组之间TNF-β表达水平的高低.结果 术前两组之间肿瘤体积大小和TNF-β的mRNA表达水平差异均无统计学意义(P＞0.05),术后30 d实验组肿瘤体积(18.44±3.46)明显小于对照组(23.36±4.11),差异有统计学意义(P＜0.05)实验组血液中TNF-β的mRNA的表达(0.58±0.18)明显高于对照组(0.47±0.14),差异均有统计学意义(P＜0.05).60 d时两组间TNF-β的mRNA表达差异无统计学意义(P＞0.05).结论 TNF联合TACE对HCC疗效好于单一TACE治疗,其同时可以刺激外周TNF-β的表达发挥更好的抗肿瘤作用.

abstractsObjective To evaluate the efficacy of TAcE combined with TNF-β in the treatment of HCC and its effects on the expression of TNF-β mRNA.Methods The HCC patients were divided into TACE+TNF-β group(group A)and TACE group(group B).The tumor size was measured by SCT.The expression of TNF-β mRNA was detected by RT-PCR in all cases of HCC,and the PCR products were tested by gel electrophoresis.The expression of TNF-β mRNA Was semi-quantified by calculating the ratio of the gray value of bands.Results The tumor size and the expression of TNF-β mRNA had no statistically significant difference before operation between two groups(P＞0.05).On the 30th day after operation,the tumor size in groups A Was(18.44±3.46),significantly less than in group B(23.36±4.11,P＜0.05);The expression of TNF-β mRNA in group A Was(0.58±0.18),significantly hisber than in group B(0.47±0.14,P＜0.05).On the postoperative day 60,there Was no significant difference in the expression of TNF-β mRNA between two groups(P＞0.05).Conclusion The curative effectiveness of TACE combined with TNF-β in the treatment of HCC is better than TACE alone.and TACE combined with TNFβ Can exert anti-tumor effects by inducing the expression of TNF-β.