摘要目的 检测胆管癌和癌旁0.5 cm胆管组织及手术切缘的正常胆管组织中CDC25A蛋白及mRNA的表达,探讨其在胆管癌发生发展中的作用.方法 应用流式细胞分析术检测58例胆管癌和正常胆管组织中CDC25A蛋白的表达,同时应用逆转录-聚合酶链反应(RT-PCR)技术检测44例术中所取的新鲜的胆管癌、同个体癌旁胆管及其手术切缘正常胆管组织中CDC25A mRNA的表达,并与临床资料进行相关分析.结果 正常胆管组织、癌旁组织、癌组织中CDC25A mRNA相对表达量分别为0.3832±0.1440、0.4550±0.1744、0.5802±0.4188,CDC25A mRNA在三组之间表达呈上升趋势(P＜0.05).CDC25A蛋白表达趋势同其相对应的基因表达趋势一致,即CDC25A的mRNA在胆管癌组织中高表达(P＜0.05).结论 CDC25A基因转录和蛋白的表达可能参与了胆管癌的发生发展过程,检测CDC25A的表达可能有助于阐明胆管癌的发生发展机制.

abstractsObjective To detect the express of CDC25A protein and gene in cholangioncarcinoma and to elucidate its role. Methods The express of CDC25A protein was detected by FCM in 58 cases of cholangioncarcinoma and normal bile duct, and the express of CDC25A gene was detected in 44 cases of cholangioncarcinoma,adjacent to eholangioncarcinoma and normal bile duct by RT-PCR. The correlation was analysed statistically between the expression of CDC25A protein and mRNA and chnicopathological parameters in cholangioncarcinoma. Results The express of CDC25A mRNA in normal bile duct, adjacent to cholangioncarcinoma, cholangioncarcinoma respectively was 0. 3832 ± 0. 1440,0. 4550 ± 0. 1744, 0.5802±0. 4188, The express of CDC25A mRNA was higher in cholangioncarcinoma than others (P ＜0.05). The protein of CDC25A in cholangioncarcinoma was higher than normal bile duct (P ＜ 0.05). Conclusion cdc25a gene transcription and protein expression is mostly like involved in the proliferation and malignant transformation of bile epithelial and then malignant progression of bile duct carcinomas. The detection of cdc25a expression may elucidate the carcinogenesis of bile duct.