摘要目的 探讨UNC-112相关蛋白1(URP1)基因在结肠癌发生发展中的意义.方法 应用定量聚合酶链反应(PCR)技术检测15例结肠癌中URP1 mRNA表达,应用组织芯片和免疫组织化学技术检测203例结肠癌中URP1蛋白表达.结果 URP1 mRNA在66.67%(10/15)的结肠癌中较相应正常结肠组织表达上调2倍以上.URP1蛋白在结肠癌中高表达率显著高于相应正常结肠组织(60.10%比14.78%).URP1蛋白表达增高与结肠癌淋巴结转移(P<0.01)、AJCC分期(P<0.01)显著相关.结论 URP1基因表达增高可能是结肠发生发展过程中的重要分子事件.

abstractsObjective To identify the expression of URP1 in colon cancer and to explore its potential role in carcinogenesis and progression of colon cancer. Methods URP1 mRNA expression was detected in 15 cases of colon cancer and the corresponding para-cancerous tissue by real-time polymerase chain reaction (PCR). By using immunohistochemistry URP1 protein expression was assayed on tissue arrays inculding the specimens of 203 patients with colon cancer. Results URP1 mRNA was upregulated more than 2-fold in 66. 7% ( 10/15 ) patients with colon cancer. Immunohistochemistry revealed that the positive expression rate of URP1 protein was 60. 10% in colon carcinoma, significantly higher than that in cancer-adjacent tissues ( 14. 78% ). Overexpression of URP1 was significantly related with lymph node metastasis (P<0.01), AJCC stage (P<0.05). Conclusion URP1 overexpression may be an important molecular event in the progression of colon carcinoma.