摘要目的 观察尿胰蛋白酶抑制剂(UTI)对人肝癌细胞株SK-HEP-1迁移和侵袭等肿瘤生物学特性的影响.方法 噻唑蓝(MTT)比色法检测UTI对SK-HEP-1细胞增殖的影响；Transwell法检测不同浓度UTI对细胞迁移和侵袭能力的作用；逆转录-聚合酶链反应(RT-PCR)和Western blot检测不同浓度UTI作用后,尿激酶型纤溶酶原激活物(UPA)的mRNA和蛋白表达.结果 UTI组对SK-HEP-1细胞的增殖无影响(P＞0.05)；与对照组比较,不同浓度的UTI组均能抑制人肝癌细胞SK-HEP-1的体外迁移和侵袭能力,且呈剂量效应关系,差异有统计学意义(P＜0.05)；不同浓度的UTI组中SK-HEP-1细胞UPA的mRNA和蛋白表达均明显下降,且呈剂量依赖性,与对照组比较差异有统计学意义(P＜0.05).结论 UTI能抑制人肝癌细胞SK-HEP-1迁移与侵袭,其机制可能与UTI抑制SK-HEP-1细胞UPA表达有关.

abstractsObjective To investigate the effects of urinary trypsin inhibitor (UTI) on the proliferation,migration and invasion of SK-HEP-1 hepatocellular carcinoma cells in vitro.Methods Effects of UTI on SK-HEP-1 cells proliferation was evaluated by methyl thiazol tetrazolium (MTT) assay; Effects of migration and invasion by UTI on SK-HEP-1 cells was evaluated by Transwell assay ; The expression of urokinase type plasminogen activator (UPA) after UTI administration was evaluated by reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blotting.Results UTI did not affect the proliferation of SK-HEP-1 cells ( P ＞ 0.05 ).UTI suppressed the migration and invasion of SK-HEP-1 cells dose-dependantly(P ＜ 0.05).UTI suppressed UPA expression of SK-HEP-1 on mRNA and protein level dose-dependantly(P ＜ 0.05),as compared with the control group.Conclusion UTI can suppress the migration and invasion of SK-HEP-1 hepatocellular cells.Decreasing the expression of UPA by UTI may be relevant to the mechanism of the migration and invasion suppression on SK-HEP-1 cells by UTI.