摘要目的 观察微小RNA(miRNA)在结肠慢传输型便秘(STC)病变结肠组织和正常结肠组织中的差异表达.方法 收集6例STC病变结肠组织和正常结肠组织标本,应用miRNA芯片检测miRNA在STC病变结肠组织和正常结肠组织中的表达.结果 5种miRNA在STC病变结肠组织正常结肠组织中的表达差异有统计学意义(P＜0.05).与正常结肠组织比较,miR-20b、miR-27b、miR-30b、miR-128及miR-129-3p在STC结肠组织中表达下调,统计学组间差异倍数均在1.2倍以上,其中miR-128及miR-129-3p下调明显,统计学组间差异倍数大于2倍.结论 部分miRNA在STC结肠组织和正常结肠组织中存在差异表达,可能参与了STC发生的分子机制.

abstractsObjective To explore the microRNA (miRNA) differential expression in the patients with colonic slow transit constipation (STC),and to provide the evidence that miRNA may be involved in the molecular pathogensis of STC.Methods Six colonic STC tissue samples and 6 normal coloic tissue samples were obtained surgically.miRNA expression was assessed using miRNA microarray.Results miRNA microarray identified five miRNA,(miR-20b,miR-27b,miR-30b,miR-128 and miR-129-3p),with down-regulated expression ( over 1.2-fold lower) in colonic STC tissues compared to normal colonic tissues (P ＜ 0.05).miR-128 and miR-129-3p were more than 2-fold decreased respectively.Conclusion Some miRNA are expressed differently between colonic STC tissues and normal colonic tissues,which may be involved in the pathogensis of STC.