摘要目的 探讨肾透明细胞癌中原癌基因胰岛素样生长因子2(IGF-2)表达与IGF-2外显子9 CpG岛甲基化状态的关系.方法 检测43例肾透明细胞癌及相应正常组织的IGF-2外显子9甲基化状态及IGF-2基因表达.结果 癌组织中IGF-2外显子9甲基化率为15.00％,正常组织为96.77％,两者比较差异有统计学意义(P＜0.01),并且与肿瘤大小、病理分级及临床分期明显相关(P＜0.05).肾癌组织中外显子9去甲基化组IGF-2双等位基因表达率显著高于甲基化组(P＜0.01).结论 IGF-2外显子9去甲基化在肾透明细胞癌的发生发展中具有重要作用,可作为反映其生物学行为的指标之一.

abstractsObjective To investigate the correlation of methylation status at exon 9 CpG island of insulin-like growth factor-2 (IGF-2) with its expression in renal clear cell carcinoma ( RCC ).Methods All the 43 RCC samples and corresponding normal tissues were analyzed for the exon 9 methylated status of IGF-2 and its expression.Results Methylation rate of exon 9 was 15.00％ in RCC group and 96.77 ％ in normal tissue respectively (P ＜0.01 ) and the methylation status of exon 9 was associated with size,stage and grade of RCC (P ＜ 0.05).The biallelic expression rate of IGF-2 in unmethylated RCC tissues was significantly higher than that in methylated RCC tissues (P ＜0.01 ).Conclusion The exon 9 unmethylated status may play an essential role in renal carcinogenesis and serve as a marker for reflecting its biological behavior.