摘要目的 探讨胃癌凋亡相关蛋白表达与体外化疗药敏性的关系. 方法 对64例胃癌标本进行Survivin、R淋巴细胞/白血病-2(bcl-2)、bax免疫组织化学染色,并以噻唑蓝(MTT)比色法检测体外化疗药物敏感性.结果 肿瘤Survivin、bcl-2、bax阳性表达率分别为90.6％、75.0％、68.8％；Survivin与bax、bcl-2与bax 间表达强度均呈负相关(r=-0.45044、-0.414 03,P＜0.01).在耐药因子表达程度与药物对肿瘤细胞抑制率的关系中,Survivin强表达时,表阿霉素(eADM)、顺铂(DDP)对肿瘤细胞的抑制率明显降低(P＜0.05),但奥沙利铂(L-OHP)对肿瘤细胞的抑制率明显增加(P＜0.05)；bcl-2强表达时,5-氟尿嘧啶(5-Fu)、紫杉醇(PTX)、eADM对肿瘤细胞的抑制率明显低于弱表达组(P＜0.05)；bax强表达组中,5-Fu、eADM、L-OHP和甲氨喋呤(MTX)对肿瘤细胞的抑制率明显高于弱表达组(P＜0.05).结论 胃癌凋亡相关蛋白表达与部分化疗药物敏感性有关.

abstractsObjective To investigate the relationship between expression of apoptosis-related proteins and chemosensitivities in gastric carcinoma.Methods The expression levels of Survivin,B lymphocytes/leukemia-2 (bcl-2) and bax were determined immunohistochemically,and the chemosenisitivities of 9 drugs were measured by nethyl thiazol tetrazolium (MTT) assay in 64 tissue specimens of gastric carcinomas.Results The positive rate of Survivin,bcl-2 and bax was 90.6％,75.0％ and 68.8％ respectively.There was negatively significant correlations hetween the expression of Survivin and bax,and bcl-2 and bax (r =-0.45044,-0.41403,both P ＜ 0.01 ). In terms of relationship between the expression of Survivin,bcl-2,bax and inhihition rate of tumor cells,the inhibition rate for epirubicin (eADM) and cisplatin (DDP) in Survivin strong expression group was lower than that in weak group (both P ＜0.05 ),but for oxaliplatin (L-OHP) the inhibition rate was increased ( P ＜0.05).The inhibition rate to 5-fluorouraeil (5-Fu),paclitaxel (PTX) and eADM was significantly lower in the bcl-2 strong expression group than the weak group (all P ＜0.05 ).There was higher inhibition rate for 5-Fu,eADM,L-OHP and methotrexate (MTX) in bax strong expression group ( all P ＜ 0.05 ).Conclusion The expression of apoptosis-related proteins in gastric carcinoma was associated with the chemosensitivities of parts of drugs.