摘要目的 观察N-(3,4-二甲氧基肉桂酰)邻氨基苯甲酸(3,4-DAA)对大鼠肝移植急性排斥反应的预防作用.方法 以Wistar大鼠为供体,SD大鼠为受体,建立大鼠肝移植模型,随机分为4组.A组:对照组(n=22),B组:3,4-DAA 400 mg/(kg·d)组(n=22),C组:环孢素A(CsA)5 mg/(kg·d)组(n=22).D组:3,4-DAA 400 mg/(kg·d) +CsA 5 mg/(kg·d)组(n=22).各组于手术当天起连续口服给药,分别于术后第3、5、7天处死受体,收集血液行肝功能检测并观察肝组织病理学变化.各组另随机选4只受体观察生存时间.结果 (1)4组受体平均生存时间分别为(11.3±1.7)、(16.0±0.8)、(21.5±1.7)、(26.5±1.3)d,B组与A组比较生存时间延长,差异有统计学意义(P＜0.01)；D组受体存活时间最长,与A、B、C3组比较,差异均有统计学意义(P＜0.0l).(2)B组大鼠术后各时间外周血谷丙转氨酶(ALT)、谷草转氨酶(AST)、总红胆素(T-BIL)值均低于A组(P＜0.05).D组各时间点ALl、AST、T-BIL值低于A、B、C组(P＜0.05).(3)术后第7天肝脏排斥活动指数B组明显低于A组(P＜0.05),D组得分最低,与A、B、C组比较,差异有统计学意义(P＜0.05).结论 3,4-DAA能抑制大鼠肝移植术后急性排斥反应,与CsA合用具有协同作用.

abstractsObjective To investigate the role of N-(3',4'-dimethoxycinnamonyl) anthranilic acid (3,4-DAA) in the prevention of acute rejection after orthotopic liver transplantation in rats.Methods The Wistar-SD rat orthotopic liver transplantation model was established and divided into four groups:group A (control group),given normal saline (2 ml/d) orally after operation; group B,given 3,4-DAA at dose of 400 mg/(kg·d) ; group C,given cyclosporin A (CsA) at 5 mg/(kg·d) ; group D,given 3,4-DAA at 400 mg/(kg· d) and CsA at 5 mg/(kg· d).The samples (peripheral blood and liver tissue) were obtained at 3rd,5th and 7th day post-operation.Pathological study and serum test were performed on each sample.Four recipients were chosen randomly in each group for survival observation.Results (1) The average survival time of recipients in groups A,B,C and D was (11.3 ± 1.7),(16.0 ±0.8),(21.5 ± 1.7) and (26.5 ± 1.3) days,respectively.Recipients in group B survived longer than in group A (P ＜0.01),and those in group D survived the longest in four groups (P ＜ 0.01) ; (2) In group A,alanine aminotransferase (ALT),aspartate aminotransferase (AST) and total bilirubin (T-BIL) were dramatically increased,and the pathological examination showed severe acute rejection (AR) on the day 7.In group B,ALT,AST and T-BIL were decreased and the pathological examination showed lower RAI on the day 7 (P ＜0.05).In group D,ALT,AST and T-BIL were decreased to the normal levels and AR was uncertainly detected on the day 7 (P ＜0.05).Conclusion 3,4-DAA exerts the anti-rejection effect after liver transplantation in rats.