摘要目的 观察肾癌石蜡标本中TMEFF2基因启动子甲基化状态,及其与各项临床指标的关系.方法 采用甲基化特异性聚合酶链反应(MSP)方法,探讨42例肾透明细胞癌石蜡标本中TMEFF2启动子甲基化与临床指标如肿瘤分期、预后等的关系.结果 42例肾透明细胞癌石蜡标本中,TMEFF2启动子甲基化状态(17例为非甲基化,13例为部分甲基化,12例呈甲基化)；各组之间T分期(非甲基化组:T1期14例、T2期2例、T3期1例、T4期0例；部分甲基化组:T1期4例、T2期4例、T3期3例、T4期2例；甲基化组:T1期2例、T2期10例、T3期例、T4期0例；)、AJCC肾癌分期(非甲基化组:Ⅰ期12例、Ⅱ期1例、Ⅲ期0例、Ⅳ期4例；部分甲基化组:Ⅰ期2例、Ⅱ期4例、Ⅲ期2例、Ⅳ期5例；甲基化组:Ⅰ期1例、Ⅱ期10例、Ⅲ期l例、Ⅳ期0例)、总胆汁酸[TBA,非甲基化组(7.43±4.59) μmol/L,部分甲基化组(4.44±1.75) μmol/L,甲基化组(3.97±3.57) μmol/L]、肌酐[CRE,非甲基化组(91.7± 24.8)μmol/L,部分甲基化组(109.0±22.2)μmol./L,甲基化组(82.7±19.1)μmol/L]、胆固醇[CHO,非甲基化组(4.88±0.95) mmol/L,部分甲基化组(4.78±1.14)mmol/L,甲基化组(3.97 ±0.69) mmol/L]、肿瘤直径[非甲基化组(5.44±1.96) cm,部分甲基化组(7.15 ±3.10) cm,甲基化组(8.22±2.60) cm]差异有统计学意义(P＜0.05).结论 TMEFF2可能参与了肾癌的发生、发展过程.

abstractsObjective To investigate the methylation status of transmembrane protein with epidermal growth factor (EGF)-like and two follistatin-like domains 2 (TMEFF2) in renal carcinoma cell line 786-0 and renal cell carcinoma specimen.Methods By MSP we investigated the relationship between methylation status of TMEFF2 in 42 paraffin-embedded clear cell renal cell carcinoma (RCC) specimens and their clinical features such as tumor stage,survival time.Results T stage (unmethylated group:T1:14,T2:2,T3:1,T4:0,partially methylated group:T1:4,T2:4,T3:3,T4:2:methylated group:T1:2,T2:10,T3:0,T4:0),AJCC renal cell carcinoma stage (unmethylated group:I stage:12,Ⅱstage:1,Ⅲ stage:0,Wstage:4; partially methylated group:Ⅰ stage:2,] stage:4,Ⅲ stage:2,Ⅳstage:5; methylated group:Ⅰ stage:1,Ⅱ stage:10,Ⅲstage:1,Ⅳstage:0),total bile acid [TBA:unmethylated group:(7.43 ±4.59) μmol/L,partially methylated group:(4.44 ± 1.75) μmol/L,methylated group:(3.97 ±3.57) μmol/L],creatine [CRE,unmethylated group:(91.7 ± 24.8) μmol/L,partially methylated group:(109.0 ±22.2) μmol/L,methylated group:(82.7 ± 19.1) μmol/L],cholesterol [CHO,unmethylated group:(4.88 ±0.95) mmoL/L,partially methylated group:(4.78 ± 1.14)mmol/L,methylated group:(3.97 ±0.69) mmol/L] and tumor diameter (unmethylated group:(5.44 ±1.96) cm,partially methylated group:(7.15 ±3.10) cm,methylated group:(8.22 ±2.60) cm were stastically significant among 3 groups with different methylation status of TMEFF2 (17 were unmethylated,13 were partially methylated and 12 were methylated).Conclusion Methylation of TMEFF2 probablly participate in the carcinogenesis and development of clear cell RCC and they deserve being futher investigated.