摘要目的 观察钙蛋白酶抑制剂ALLN对骨肉瘤细胞株U2OS、MG63增殖和凋亡的影响.方法 培养骨肉瘤细胞株U2OS和MG63,采用浓度分别为0.1、1.0、10.0、100.0、1 000.0μmol/L的ALLN处理,噻唑蓝(MTT)法检测ALLN对于骨肉瘤细胞株U2OS和MG63体外生长的抑制作用,流式细胞术和Western blot检测ALLN的凋亡诱导作用,克隆形成实验检测ALLN对骨肉瘤细胞株U2OS和MG63克隆形成能力的影响.结果 ALLN对于骨肉瘤细胞株U2OS和MG63有明显的生长抑制作用,U2OS和MG63的半数抑制浓度均约为10 μmol/L,ALLN作用于骨肉瘤细胞株U2OS和MG63后可明显诱导其细胞凋亡,同时ALLN也可降低两种细胞的克隆形成能力.结论 ALLN可明显抑制骨肉瘤细胞株的增殖,促进细胞凋亡.

abstractsObjective To investigate the effect of calpain inhibitor ALLN on osteosarcoma cell proliferation and apoptosis.Methods Osteosarcoma cell line U2OS and MG63 were treated with 0.1,1.0,10.0,100.0,1000.0 μ mol/L of ALLN,cell proliferation was measured by methyl thiazol tetrazolium(MTT),cell apoptosis was measured by flow cytometry and Western blotting,clone formation assay was performed to study the effect of ALLN on osteosarcoma cell clone formation ability.Results ALLN suppress U2OS and MG63 cell proliferation with an IC50 about 10 μmol/L,ALLN promotes the apoptosis of U2OS and MG63.Morever,ALLN decreases the clone formation ability of U2OS and MG63.Conclusion The calpain inhibitor ALLN suppresses osteosarcoma cell proliferation and promotes apoptosis.