摘要目的 探讨二氧化铈纳米颗粒(Nanoceria)对大鼠心肌细胞氧化损伤的影响及其机制.方法 将体外培养的大鼠心肌细胞随机分为实验组、模型组和空白组;实验组中加入浓度为10 nmol/L Nanoceria培养液培养24h,模型组、空白组用普通培养液培养24h,实验组及模型组培养液中加入浓度为25 μmol/L H2O2孵育2h,空白组不做处理.检测心肌细胞内总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)-Px、丙二醛(MDA)的含量,核因子相关因子2(Nrf2)在细胞内分布,以及Nrf2、血红素氧合酶-1(HO-1)、苯醌氧化还原酶基因(NQO1) mRNA的表达.结果 同模型组相比实验组SOD(92.05 ±7.39比74.99±10.86)、GSH-Px(15.76±2.75比10.72±1.60)、T-AOC(1.15 ±0.25比0.59±0.11)含量明显增高(P＜0.05),MDA(15.14±1.28比18.48±2.09)含量明显降低(P＜0.05);同空白组和模型组比较实验组细胞Nrf2核内转位率增加;同空白组和模型组相比较实验组Nrf2(1.700±0.270比0.930±0.146比0.610±0.102)、HO-1(2.200±0.282比1.030±0.109比0.400±0.171)、NQO1(1.920±0.161比0.980±0.133比0.490±0.257) mRNA表达明显增加(P＜0.01).结论 (1)Nanoceria对乳鼠心肌细胞的氧化损伤有一定程度保护作用.(2) Nanoceria可以诱导Nrf2核内移位及上调基因Nrf2、HO-1、NQO1 mRNA表达.(3) Nanoceria可能通过Nd2/抗氧化反应元件(ARE)信号通路保护大鼠心肌细胞免受氧化应激损伤.

abstractsObjective To study the effect of cerium oxide nanoparticles (Nanoceria) on myocardial cells oxidative damage and its possible mechanisms.Methods Myocardial cells culture in vitro were randomly divided into experimental group,model group and the blank group.The experimental group was added at a concentration of 10 nmol/L cerium oxide nanoparticles culture for 24 h,model group and the blank group with normal culture for 24 h,the final concentration of 25 μmol/L H2O2 were joined to experimental group and model group for 2 h,the blank group not treated.Detecting of total antioxidant capacity (T-AOC),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) content in myocardial cells,nuclear factor related factor 2 (Nrf2) distribution in cells and Nrf2,heine oxygenase-1 (HO-1),nicotinamide adenine dinucleotide phosphate (NADPH):quinone oxidoreductase 1 (NQO1) mRNA express.Results Compared with the model group,the experimental group SOD (92.05±7.39 vs.74.99 ±10.86),GSH-Px (15.76±2.75 vs.10.72 ±1.60) T-AOC,(1.15 ±0.25 vs.0.59 ±0.11) content were significantly increased (P ＜0.05),MDA (15.14 ± 1.28 vs.18.48 ±2.09) content decrease (P ＜ 0.05);With the blank group and the model group,the experimental group increased the Nrf2 nuclear translocation rate;Compared with the blank group and the model group,the experimental group Nrf2 (1.700 ± 0.270 vs.0.930 ± 0.146,0.610 ± 0.102),HO-1 (2.200 ± 0.282 vs.1.030 ±0.109,0.400 ±0.171),NQO1 (1.920 ±0.161 vs.0.980 ±0.133,0.490 ±0.257) mRNA expression were significantly increased (P ＜0.01).Conclusion (1) Nanoceria has a certain extent protective effect aganist oxidative damage in neonatal rat cardiomyocytes.(2) Nanoceria can induce the nuclear translocation of Nrf2 and upregulate genes Nrf2,HO-1,NQO1 mRNA expression.(3) Nanoceria maythrough the Nrf2/antioxidant response element (ARE) signal pathway to protect neonatal rat cardiomyocytes from oxidative stress injury.