摘要目的 通过分析免疫细胞社会中的肿瘤细胞与宿主巨噬细胞之间的关系,探寻肿瘤细胞社会中打破免疫平衡的大事件.方法 共培养红绿双色荧光示踪的胶质瘤干/祖细胞与宿主巨噬细胞,置于活细胞工作站实时摄影缩时成像并视频分析目标细胞的社会行为.结果 相关细胞实时动态连续观察发现:(1)细胞间传递信息的连接管道的6种类型.(2)2个活跃细胞相互作用后变成1个细胞,即细胞融合;已融合的细胞与另一个细胞再融合.(3)融合细胞的命运有对称分裂产生子细胞和瞬间凋亡两种.(4)存在一个细胞进入另一细胞内后胞释现象.结论 胶质瘤干/祖细胞与宿主巨噬细胞间连接方式、融合、分裂及胞释过程,可作为胶质瘤干/祖细胞诱导宿主巨噬细胞恶变的细胞行为学依据,对进一步理解肿瘤细胞社会成员间的复杂关系有重要意义.

abstractsObjective The roles of real-time monitoring of cytokine secretion at the single immunocyte level,using the concept of immune cells sociology have been reported recently.We analyzed the relationships between glioma stem/progenitors cells and host macrophages in the immune cells society,for the purpose of discovering the important events which broke the immune balance of the cancer cells society.Methods The dual fluorescence tracing method was applied to trace the co-cultured glioma stem/progenitor cells and host macrophages,interactions between the two kinds of cells were observed under live cell imaging system,Yellow cells in the co-culture system were mono-cloned,and proliferated in vitro.Then their social activities were observed and recorded under the live cell imaging system.Results Via real-time dynamic observation of the target cells,we noticed that:(1) there were 6 types of conjunction pipes functioned as transfering intercellular information;(2) two activating cells formed hybrid cell after mutual interactions,and the hybrid cell fused with another cell again;(3) the fates of hybrid cell were to generate offspring cells by symmetrical division,or to undergo apoptosis;(4) the phenomenon of cell in cell,which can release out another cell,namely entosis.Conclusion The research revealed the patterns of cell conjunction between glioma stem/progenitor cells and host macrophages,the processes of cell fusion,division and entosis.These were the cell sociology evidences of glioma stem/progenitor cells inducing malignant transformation of host macrophages,which offered basic knowledges exploring the complex relationships among the residents in the tumor cell society.