摘要目的 观察肝再生磷酸酶-3(PRL-3)、趋化因子(CCL26)与肿瘤相关巨噬细胞(TAMs)在结肠癌组织中的表达及对患者预后影响.方法 免疫组织化学分析结肠癌患者83例,检测Ⅰ～Ⅳ期结肠癌组织PRL-3、CCL26和TAMs表达;Kaplan-Meier法对比分析各组患者的生存情况;迁移实验分析CCL26对TAMs迁移作用.结果 免疫组织化学分析显示结肠癌患者随着肿瘤分期TNM进展,PRL-3,CCL26与TAMs逐渐升高,在Ⅰ期结肠癌中三者表达阳性率为(25％、20％、15％),在Ⅳ期肝转移灶中,三者阳性率分别为(94.7％、89.4％、78.9％,P＜0.01).Kaplan-Meier生存曲线统计分析各蛋白对患者生存影响,PRL-3、CCL26和TAMs阳性的结肠癌患者其术后总生存期明显较阴性组低(P＜0.001).迁移实验显示随着CCL26浓度递增对TAMs有明显趋化作用(P＜0.01).结论 PRL-3能促进结肠癌组织分泌CCL26,趋化TAMs聚集,促进结肠癌进展,降低患者总的生存期.

abstractsObjective To observe the phosphatase of regenerating liver-3 (PRL-3), chemokine CCL26 and tumoassociated macrophage(TAMs) expression in colorectal cancetissue and the affection on the prognosiof patients.MethodWe used immunohistochemistry assayto detecthe PRL-3, CCL26 and TAMexpression in colorectal cancetissue from 83 colorectal cancepatients.Kaplan-Meiesurvival curvewere plotted and observed the survival situation of patients.Invasion assayto determine the effecof CCL26 to promote migration of TAMs.ResultImmunohistochemistry assayshowed thathe PRL-3, CCL26 and TAMexpression in colorectal cancetissue of metastasestage (94.7％, 89.4％, 78.9％) were highethan stage Ⅰ (25％, 20％, 15％, P ＜ 0.01).Kaplan-Meiesurvival curvedemonstrated thapatientwith negative olow expressionof PRL-3, CCL26 and TAMsurvive significandy longethan those high expression(P ＜0.01).Transwell invasion assayshowed thathe migration of TAMwaenhanced in concentration-dependenmannewhen TAMcocultured with differenconcentration chemokine CCL26 (P ＜ 0.01).Conclusion PRL-3 promotecolorectal cancetissue to secrete chemokine CCL26, enhancetumor-associated macrophagemigration, and reducethe patients' overall survival.