摘要目的 探讨兔椎间盘生理屏障损伤后辅助性T细胞1 (Th1)/辅助性T细胞2(Th2)平衡漂移状态.方法 采用纤维环穿刺构建兔椎间盘退变的动物模型,在术后1、3d、1、3周采集外周静脉血,采用双抗夹心酶联免疫吸附测定(ELISA)方法,分别检测血清中Th1、Th2型细胞因子水平[肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、白细胞介素(IL)-10及IL-4].结果 造模后的1、3d、1、3周兔血清中TNF-α含量分别为(150.531±56.74)、(83.833±37.434)、(90.990±3.554)、(87.950±13.289) ng/L;IFN-γ含量分别为(99.263±18.421)、(125.427±9.156)、(180.368±18.293)、(165.531±12.548) ng/L,明显高于造模前(P＜0.05),术后各个时间点模型组血清中TNF-α与IFN-γ含量高于假手术组与正常组(P＜0.05).造模后的1、3d、1、3周兔血清中IL-10含量分别为(33.947±3.164)、(27.928±0.493)、(29.992±4.33)、(30.025±2.339) ng/L;IL-4含量分别为(34.517±3.032)、(35.056±1.882)、(35.983±2.369)、(32.549±5.086) ng/L,与术前比较未见明显增高(P＞0.05).术后各个时间点模型组血清中IL-10与IL-4含量与假手术组及正常组比较差异无统计学意义(P＞0.05).结论 生理屏障损伤后椎间盘发生退变的机制与Th1优势状态有关.

abstractsObjective To explore the state of helper T cell 1 (Th1) / helper T cell 2 (Th2) balance drift after physiological barrier injury of rabbit intervertebral disc.Methods A rabbit model of intervertebral disc degeneration was established by anular puncture.Peripheral venous blood was collected at 1st,3rd,7th,and 21st day after surgery.Serum levels of Th1/Th2 cytokines [tumor necrosis factor (TNF)-α,interferon (IFN)-γ,interleukin (IL)-10 and IL-4] were determined by ELISA.Results At 1 st,3rd,7th,and 21st day after surgery,the serum TNF-α level was (150.531 ± 56.74),(83.833 ± 37.434),(90.990 ± 3.554) and (87.950 ± 13.289) ng/L respectively,and serum IFN-γ level was (99.263 ±18.421),(125.427 ±9.156),(180.368 ±18.293) and (165.531 ±12.548) ng/L respectively,which were markedly increased after modeling (P ＜ 0.05 or P ＜ 0.01).At 1 st,3rd,7th,and 21st day after modeling,the serum IL-10 level was (33.947 ±3.164),(27.928 ±0.493),(29.992 ±4.33)and (30.025 ± 2.339) ng/L,and serum IL-4 level was (34.517 ± 3.032),(35.056 ± 1.882),(35.983 ±2.369) and (32.549 ± 5.086) ng/L respectively.As compared with preoperation,the serum IL-10 and IL-4 levels in three groups after operation were not significantly increased (P ＞0.05).There was no significant difference in serum IL-10 and IL-4 levels among three groups (P ＞0.05).Conclusion Mechanism of intervertebral disc degeneration after physiological barrier injury may be related to Th1 state.