摘要目的 探讨肝细胞癌中清道夫受体A类5型(SCARA5)蛋白表达与临床病理特征及其基因启动子甲基化状态间的相关性.方法 应用组织芯片技术检测112例肝细胞癌(HCC)及癌旁组织中SCARA5的蛋白表达,分析其表达与临床病理特征间的关系;并应用甲基化特异性聚合酶链反应(MSP)检测其基因启动子甲基化状态.结果 HCC中低或无SCARA5蛋白表达率为77.7％(87/112),而癌旁组织为25.0％ (28/112),差异有统计学意义(P＜0.05);HCC中其表达水平与性别、年龄、乙型病毒性肝炎、肝硬化、肿瘤包膜等临床病理特征间无明显相关(P＞0.05),而与肿瘤大小、血管侵犯、分化程度以及临床分期等因素间明显相关(P＜0.05);HCC中SCARA5基因甲基化率为60.7％,明显高于癌旁组织(11.6％,P＜0.05),并且甲基化与其蛋白表达呈负相关(r=-0.196,P ＜0.05).结论 SCARA5低表达可能对HCC的发生发展具有重要作用,并且甲基化可能是导致其低表达的原因之一.

abstractsObjective To investigate the expression and clinical significance of Scavenger receptor class A,member 5 (SCARA5) in patients with hepatocellular carcinoma (HCC),and to explore the relationship between methylation status of SCARA5 gene promoter and its protein expression.Methods SCARA5 expression in tumor and matched adjacent non-cancerous liver tissues from 112 patients with HCC was deteced by immunohistochemistry using tissue microarray technology.The relationship between the protein expression of SCARA5 and the clinical,pathological features in patients with HCC was also analyzed.The promoter methylation patterns of SCARA5 gene in tumor and matched adjacent non-cancerous liver tissues were also measured by methylation-specific PCR technique.Results Negative and weak staining of SCARA5 was observed in 77.7％ (87/112) of the HCC tissue samples,contrast to 25.0％ (28/112) in the adjacent non-cancerous liver tissue samples.SCARA5 was significantly decreased in HCC tissues (P ＜ 0.01).The expression of SCARA5 was not obviously related to sex,age,hepatitis B virus (HBV) status,liver cirrhosis,or capsular invasion (P ＞ 0.05).However,the expression of SCARA5 was significantly correlated with tumor size,vascular invasion,Edmondson-Steiner grade and TNM stage (P ＜ 0.05).Methylation of SCARA5 gene promoter was found in 60.7％ of the all tumor tissue samples.However,methylation was only found in 11.6％ of the adjacent non-tumor samples,which was significantly lower than that in tumor tissue samples (P ＜ 0.05).Meanwhile,the expression of SCARA5 in tumor tissue samples was negatively correlated with promoter methylation status (P ＜ 0.05).Conclusion It suggests that the decreased expression of SCARA5 may have an important role in the development of HCC,and the promoter methylation of SCARA5 gene may be one of the reasons for the decreased expression of SCARA5.