摘要目的 对肾动脉、尾静脉、包膜下3种间充质干细胞(MSCs)移植方式的归巢率.方法 比较通过可复性左侧输尿管梗阻的大鼠模型(R-UUO)模拟临床急性肾梗阻的病变.4周龄雌性SD大鼠,无特定病原体(SPF)级,30只,随机数字表法分为3组,每组每个时间点各5只,采用经肾动脉、尾静脉、包膜下3种方式移植荧光标记的骨髓间充质干细胞[1,1'-双十八烷基-3,3,3',3'-四甲基羰花青碘化物(DiR)-BMSCs],结合小动物活体成像系统(IVIS)对移植后1、48 h细胞在体内的归巢进行观察及分析.结果 MSCs不同移植方式在梗阻侧肾脏的早期归巢率分别为:肾动脉组为(9.59×105±5.26×105)/g,包膜下组为(17.65×105±4.77×105)/g,尾静脉组为(2.18×105±0.45×105)/g,3组比较差异有统计学意义(P＜0.05).结论 3种移植途径的早期移植效率由高到低依次为包膜下移植、肾动脉移植、尾静脉移植.实际应用中,要根据具体情况选择合理的移植方式.

abstractsObjective To evaluate and compare the homing of MSCs (Mesenchymal Stem Cells) transplanted through three routes:renal artery,tail vein and subcapsular.Methods It used an animal model of reversible unilateral ureteral obstruction (R-UUO) to simulate clinical acute renal obstruction.Thirty SPF femal SD rats (4w) were divided into three groups.Every group contained 5 rats at each time point;MSCs labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) were transplanted into thereversible unilateral ureteral obstruction (R-UUO) animals through three routes:renal artery,tail vein and subcapsular.Homing of MSCs was evaluated by in vivo imaging system (IVIS).Resalts The homing efficiency of the three delivery routes is:renal artery (9.59 ×105 ±5.26 ×105) cells/g,subcapsular (17.65 × 105 ±4.77 × 105) cells/g,tail vein (2.18 × 105 ±0.45 × 105) cells/g.It was significantly different at the early stage (P ＜ 0.05).Conclusion The homing efficiency of MSCs transplanted through the three routes is:subcapsular,renal artery,tail vein,from high to low.Although the transplantation efficiency of the three delivery routes was the same as the results of MSCs homing efficiency,it need to choose a reasonable route according to actual condition in practical application.