摘要目的 观察脑胶质瘤中微小RNA(miRNA,miR)-105的表达,探讨其对肿瘤细胞增殖、迁移等生物学行为的影响.方法 收集不同病理级别的标本,采用实时定量聚合酶链反应(Real-time PCR)检测脑胶质瘤及癌旁组织中miR-105的表达.细胞计数试剂盒(CCK-8)实验检测细胞增殖.Transwell实验检测细胞迁移能力.结果 与癌旁组织比较,miR-105在脑胶质瘤和癌旁组织中的表达量分别为6.043±0.427与5.216±0.418,脑胶质瘤组织中miR-105的表达明显下调为癌旁组织中miR-105表达量的56.37%,差异有统计学意义(P=0.000),miR-105在脑胶质瘤组织中的表达水平显著低于正常组织,且与病理分级呈负相关(P=0.001).CCK-8实验中miR-105 mimic 转染后细胞增殖速度较未处理组下降0.365±0.033(50.65%,P=0.002),抑制miR-105后,增殖速度较未处理组升高(2.434±0.030,54.76%,P=0.021),差异有统计学意义.Transwell 实验中,miR-105转染后细胞迁移能力明显降低 (P=0.009).结论 miR-105在脑胶质瘤中呈低水平表达,且与胶质瘤级别的升高呈负相关,还可以抑制脑胶质瘤的增殖、迁移等生物学行为.

abstractsObjective To investigate the microRNA-105 (miR-105) expression level in brain glioma and the role of miR-105 in the progression and metastasis of brain glioma and assess its value in predicting the prognosis of brain glioma patients.Methods Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of miR-105 gene in brain glioma and peri-cancerous tissues.The miR-105 mimic and miR-105 inhibitor were transfected to U87 cells respectively.Cell proliferation was analyzed by cell counting assay kit-8 (CCK-8).Cell invasion assays was analyzed by Transwell assay.Results Real-time PCR showed that the miR-105 expression in human brain glioma tissues was lower than that in non-cancerous brain tissues (6.043±0.427 vs.5.216±0.418) The expression of miR-105 in brain glioma was reduced to 56.37% of that in peri-cancerous tissues (P=0.000), and was negatively associated to tumor grade (P=0.001).The results of CCK-8 proliferation assay showed that cell proliferation speed was reduced by 0.365±0.033 (50.65%, P=0.002) in miR-105 mimics-transfected cells as compared with that in the untreated cells, and after inhibiting miR-105, the speed was increased by 2.434±0.030 (54.76%, P=0.021).The invasion of the cells that were transfected with the miR-105 mimic was dramatically decreased as compared with the scrambled control and untreated cells (P=0.009).Conclusion miR-105 functions as a tumor suppressor miRNA in brain glioma.The expression of miR-105 is aberrantly low in glioma tissues.Transient transfection of miR-105 mimics leads to decreased proliferation and progression in U87 glioma cells.miR-105 plays an important role in the development of brain glioma.