摘要目的 探讨人Stomatin样蛋白2(SLP-2)基因调控Wnt/β-连环蛋白(β-catenin)信号通路对胃癌细胞增殖凋亡的影响及机制.方法 Western blot检测SLP-2基因在人胃癌组织中的表达;将NC-小干扰RNA(siRNA)、SLP-2-siRNA1、SLP-2-siRNA2转染人胃癌SGC-7901细胞,未转染任何siRNA的作为对照组,Western blot检测转染后各组细胞中SLP-2蛋白表达;细胞转染48h后,流式细胞仪检测细胞凋亡;Western blot检测活化的的Cleaved含半胱氨酰的天冬氨酸特异性蛋白酶-3(Caspase-3)、β-catenin、c-Myc、细胞周期蛋白D1(Cyclin D1)蛋白表达.结果 胃癌组织中SLP-2的蛋白表达(0.283±0.016)显著高于癌旁组织(0.082±0.016)(t=7.789,P=0.003);SLP-2-siRNA2组的抑制效果更明显,选择作为后续研究;LP-2-siRNA组(16.62±1.32)%细胞凋亡率及Cleaved Caspase-3蛋白表达(1.421±0.210)均显著高于对照组(1.56±0.56)%、(0.510±0.083),β-catenin(0.157±0.024)、c-Myc(0.039±0.016)、Cyclin D1(0.052±0.012)蛋白表达显著低于对照组(0.311±0.036、0.137±0.026、0.183±0.021)(t凋亡率=9.324,P凋亡率=0.001;tβ-catenin=8.868,Pβ-catenin=0.003;tc-Myc=9.112,Pc-Myc=0.002;tCyclin D1=9.987,PCyclin D1=0.001).结论 SLP-2在胃癌中高表达,沉默SLP-2的表达能抑制人胃癌SGC-7901细胞增殖和促进细胞凋亡,其机制与Wnt/β-catenin信号通路的调控有关.

abstractsObjective To investigate the effect of stomatin-like protein 2 (SLP-2) gene on the proliferation and apoptosis of gastric cancer cells by Wnt/β-catenin signaling pathway.Methods The expression of SLP-2 gene in human gastric cancer tissues was detected by Western blotting;NC-siRNA, SLP-2-siRNA1 and SLP-2-siRNA2 were transfected into human gastric cancer SGC-7901 cells, and not transfected with any siRNA as the control group;the expression of SLP-2 protein after transfected cells was detected by Western blotting;cells transfected for 48 h, cell apoptosis was detected by flow cytometry;Cleaved Caspase-3, β-catenin, c-Myc, Cyclin D1protein expression was detected by Western blotting.Results The expression of SLP-2 protein in gastric carcinoma (0.283±0.016)was significantly higher than paracancerous tissues (0.082±0.016), (t=7.789, P=0.003);the inhibitory effect of SLP-2-siRNA2 group was more obvious, and was choiced as a follow-up study;the apoptosis rate (16.62±1.32)% of cells and Cleaved Caspase-3 (1.421±0.210) protein expression in SLP-2-siRNA group were significantly higher than the control group (1.56±0.56)%, (0.510±0.083), β-catenin (0.157±0.024), (0.039±0.016), Cyclin D1 (0.052±0.012) protein expression was significantly lower than the control group (0.311±0.036, 0.137±0.026, 0.183±0.021) (tapoptosis rate=9.324, Papoptosis rate=0.001;tCleaved Caspase-3=6.112, PCleaved Caspase-3=0.006;tβ-catenin=8.868, Pβ-catenin=0.003;tc-Myc=9.112, Pc-Myc=0.002;tCyclin D1=9.987, PCyclin D1=0.001).Conclusion SLP-2 is highly expressed in gastric cancer, Silencing SLP-2 expression can inhibit the proliferation and apoptosis of human gastric cancer cell line SGC-7901, and its mechanism is related to the regulation of Wnt/β-catenin signaling pathway.