摘要目的 建立并经鉴定可用来监测贴壁细胞生长和细胞染色过程的基于芯片组装而成的无透镜显微镜组件系统.方法 通过将样本放在一个厚1 μm Si3N4基层在一个5兆像素、CMOS成像元件平面和2.2μm像素尺寸的MEMS巢式芯片上.采用样品与成像元件平面间的距离的变化,使直径≥15 μm的细胞成像.这套组件被用以培养和监测胆管癌细胞TFK-1的生长形态(2 d),并监测细胞的色素染色.结果 TFK-1细胞在培养0、24、48 h后,经组件拍摄的TFK-1细胞生长及细胞染色过程图像无论是静止细胞,还是成三角状伸展的细胞边缘影像,与4×物镜放大倍数的显微镜成像质量相当.结论 这套手提式的、经济方便的无透镜成像组件很适合用以集成一套基于细胞研究的芯片实验室系统.

abstractsObjective To establish and characterize an on-chip assembled lensless microscopy module for optical monitoring of adherent growing mammalian cells.Methods The module could image the morphology of mammalian ceils with diameters ≥ 15 μm by interfacing samples in a micro-electro-mechanical systems (MEMS) cavity chip with a 1 μm thick Si3N4 substrate onto a 5 megapixel,comlementary metal-oxide-semiconductor (CMOS) image sensor plane with 2.2 μm pixel size.The module was applied for culturing and optical monitoring the growth morphology of TFK-1 cells for 2 days and checking the colorimetric staining of cells.Results After TFK-1 cells were cultured by 0,24,48 h,all the images about TFK-1 cells at edges for both standing and triangular spreading cells and the colorimetric staining of cells taken by the lensless module were in good accordance to those obtained by a 4 × objective bright field microscope.Conclusion This portable and economical lensless imaging module is suitable to be integrated in lab-on-a-chip cell-based sensor systems.