摘要目的 观察斯钙素-1(STC-1)在膀胱癌组织中的表达及其对膀胱癌细胞5637生物学行为的影响.方法 取60例膀胱癌患者癌组织及癌旁正常组织,采用反转录-聚合酶链反应(RT-PCR)、Western blot技术检测膀胱癌及其对应的癌旁组织中STC-1 mRNA和蛋白的表达.随后分别应用细胞计数试剂盒(CCK-8)、流式细胞仪、Transwell小室观察STC-1蛋白对5637细胞增殖、凋亡及迁移能力的影响.结果 膀胱癌组织中STC-1 mRNA的相对表达量(0.826 ±0.112)明显高于癌旁正常组织(0.283 ±0.006)(P=0.000).STC-1蛋白在膀胱癌组织中的相对表达量(0.795±0.107)显著高于癌旁正常组织(0.164±0.039) (P=0.000).随着TNM分期的增加,STC-1 mRNA和蛋白表达水平有升高的趋势,但各分期间的表达差异无统计学意义.与对照组比较,STC-1可显著促进5637细胞的增殖.此外,与STC-1单克隆抗体组(56.26 ±2.27)％和空白对照组(53.43±2.04)％比较,STC-1蛋白组的5637细胞凋亡比例(18.48±0.87)％明显下降(P=0.000).Transwell 小室结果显示,STC-1蛋白组细胞迁移数为(96±7)个,与STC-1单克隆抗体组[(28±2)个]和空白对照组[(32±3)个]比较,细胞迁移能力明显增强(P =0.000).结论 STC-1可能在膀胱癌的发生发展中发挥重要作用.

abstractsObjective To explore the expression of stanniocalcin-1 (STC-1) in bladder cancer and the effects of STC-1 on the biological behaviors of bladder cancer cell line 5637.Methods Cancer tissues and adjacent normal tissues were collected from 60 patients with bladder cancer.Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting were applied to investigate the expression of STC-1 in bladder cancer tissues and paracancerous tissues.Cell counting kit-8 (CCK-8) assay,flow cytometry and Transwell assay were used to observe the effects of STC-1 protein on the proliferation,apoptosis and migration of 5637 cells.Results The relative expression level of STC-1 mRNA was (0.826 ±0.112) in bladder cancer tissues,which were over-expressed as compared with that in the paracancerous tissues (0.283 ± 0.006) (P =0.000).The relative expression level of STC-1 protein was (0.795 ± 0.107) in bladder tissues,which was up-regulated as compared with that in the paracancerous tissues (0.164±0.039) (P =0.000).In addition,although the level of STC-1 mRNA and protein increased with the higher clinical stages,there was no statistically significant difference.As compared with control group,STC-1 could promote the proliferation of 5637 cells.In addition,as compared with STC-1 monoclonal antibody group [(56.26 ± 2.27) ％] and blank control group [(53.43 ± 2.04) ％],the cell apoptosis ratio in STC-1 group [(18.48 ± 0.87) ％] decreased significantly (P =0.000).Transwell assay revealed that as compared with STC-1 monoclonal antibody group [(28 ± 2) cells] and blank control group [(32 ±3) cells],the number of migrating cells in STC-1 group [(96 ±7)cells] was significantly increased (P =0.000).Conclusion Our results suggested that STC-1 might play a key role in the development of bladder cancer.