摘要目的 探讨泛素结酶E2s(UBE2S)在胆囊癌中表达及临床意义.方法 应用免疫组织化学检测UBE2S在85例胆囊癌、28例胆囊癌前病变及15例胆囊良性病变蜡块组织表达,以Western blot检测胆囊良恶性病变新鲜组织加以验证,并分析其临床意义.结果 UBE2S在胆囊癌组织中表达显著高于胆囊癌前病变[染色指数(SI):4.082±3.413比2.607±2.025,P=0.021]和良性病变(SI:4.082±3.413比2.067±1.335,P=0.001),在新鲜胆囊癌组织中表达亦高于良性病变(Western blot灰度值:0.734 ±0.181比0.197±0.099,P=0.002).UBE2S表达与肿瘤分化程度(x2=6.978,P=0.031)、肝转移(x2=8.155,P=0.004)显著相关.UBE2S表达(P =0.024)和肿瘤分化程度(P=0.000)、Nevin分期(P =0.040)、R0切除(P=0.011)是影响胆囊癌患者预后的独立因素;UBE2S高表达胆囊癌患者生存期较短(P =0.011).结论 UBE2S高表达是胆囊癌患者不良预后的独立因素.

abstractsObjective To investigate the expression of ubiquitin-conjugating enzyme E2s (UBE2S) in gallbladder cancer (GBC) tissues and its clinical significance.Methods The expression of UBE2S was examined in 85 GBC,28 pre-malignant lesion and 15 benign lesion tissues of the gallbladder by immunohistochemistry,and was verified by western blotting.The relationship between UBE2S expression and clinicopathological characteristics and prognostic factors of GBC patients was analyzed.Results Immunohistochemistry showed that UBE2S was highly expressed in GBC tissues compared to pre-malignant lesion tissues [stain index (SI):4.082 ± 3.413 vs.2.607 ± 2.025,P =0.021)] and benign lesion tissues (SI:4.082 ± 3.413 vs.2.067 ± 1.335,P =0.001),and western blotting showed that UBE2S protein was highly expressed in GBC tissues compared to benign lesion tissues (grey-scale value:0.734 ±0.181 vs.0.197 ± 0.099,P =0.002).UBE2S was positively correlated with tumor differentiation degree (x2 =6.978,P =0.031) and liver metastasis (x2 =8.155,P =0.004).Cox model showed that UBE2S expression (P =0.024) as well as tumor differentiation degree (P =0.000),Nevin stage (P =0.040) and R0 resection (P =0.011) were prognostic factors of GBC patients.High UBE2S expression patients with GBC have a shorter survival time (P =0.011).Conclusion High UBE2S expression is an independent factor of unfavorable prognosis in GBC patients.